EVALUATION OF RAPID QUANTITATIVE LATEX ASSAY TO DETECT NEWCASTLE DISEASE VIRUS IN CHICKE

• Main Authors: K .P. Deepa, P.M Priya, M. Mini
• Format: Article
• Language: English
• Published: Director of Academics and Research, Kerala Veterinary and Animal Sciences University 2017-07-01
• Series: Journal of Veterinary and Animal Sciences
• Subjects: newcastle disease virus; haemagglutination; haemagglutination inhibition; latex agglutination test; kerala
• Online Access: https://www.jvas.in/public_html/upload/article_file/article_file_qb2vku.pdf?t=qb2vku

The present study was undertaken to evaluatethe efficacy of a rapid quantitative latex assay to detect Newcastle disease virus (NDV) in birds. Out of 28 clinical samples collected from disease suspected birds, haemagglutination test detected 12 samples as positive and were confirmed as NDV by HI test. The latex agglutination test (LAT) was standardized and only 6 samples were found to be positive. The HA titre ranged from 1:8 to 1:128 and LAT titres were 1:2 in all LAT positive samples. Based on the statistical analysis, there exists a significant difference (p<0.05) between the two tests employed and HA-HI tests was found to be having higher sensitivity than LAT