Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR

Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR

<p>Abstract</p> <p>Background</p> <p>We have developed an ultrasensitive method based on conventional PCR preamplification followed by nested amplification through real time PCR (qPCR) in the presence of the DNA intercalating agent EvaGreen.</p> <p>Results&l...

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Bibliographic Details
Main Authors: López-Revilla Rubén, Hernández-Arteaga Socorro
Format: Article
Language:English
Published: BMC 2010-05-01
Series:Infectious Agents and Cancer
Online Access:http://www.infectagentscancer.com/content/5/1/9

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