Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR
<p>Abstract</p> <p>Background</p> <p>We have developed an ultrasensitive method based on conventional PCR preamplification followed by nested amplification through real time PCR (qPCR) in the presence of the DNA intercalating agent EvaGreen.</p> <p>Results&l...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
BMC
2010-05-01
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Series: | Infectious Agents and Cancer |
Online Access: | http://www.infectagentscancer.com/content/5/1/9 |