Native mass spectrometry reveals the initial binding events of HIV-1 rev to RRE stem II RNA

Native mass spectrometry reveals the initial binding events of HIV-1 rev to RRE stem II RNA

The HIV-1 RNA-binding protein rev facilitates nuclear export of viral RNA. Here, the authors use native mass spectrometry to study the interactions between rev-derived peptides and rev response elements of HIV-1 RNA, providing mechanistic insights into rev recognition and recruitment.

Bibliographic Details
Main Authors: Eva-Maria Schneeberger, Matthias Halper, Michael Palasser, Sarah Viola Heel, Jovana Vušurović, Raphael Plangger, Michael Juen, Christoph Kreutz, Kathrin Breuker
Format: Article
Language:English
Published: Nature Publishing Group 2020-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19144-7
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spelling doaj-13e70d04a2b44c299060a0fd63802d9e2021-05-11T09:01:11ZengNature Publishing GroupNature Communications2041-17232020-11-0111111010.1038/s41467-020-19144-7Native mass spectrometry reveals the initial binding events of HIV-1 rev to RRE stem II RNAEva-Maria Schneeberger0Matthias Halper1Michael Palasser2Sarah Viola Heel3Jovana Vušurović4Raphael Plangger5Michael Juen6Christoph Kreutz7Kathrin Breuker8Institute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of InnsbruckInstitute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of InnsbruckInstitute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of InnsbruckInstitute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of InnsbruckInstitute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of InnsbruckInstitute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of InnsbruckInstitute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of InnsbruckInstitute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of InnsbruckInstitute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of InnsbruckThe HIV-1 RNA-binding protein rev facilitates nuclear export of viral RNA. Here, the authors use native mass spectrometry to study the interactions between rev-derived peptides and rev response elements of HIV-1 RNA, providing mechanistic insights into rev recognition and recruitment.https://doi.org/10.1038/s41467-020-19144-7
collection DOAJ
language English
format Article
sources DOAJ
author Eva-Maria Schneeberger
Matthias Halper
Michael Palasser
Sarah Viola Heel
Jovana Vušurović
Raphael Plangger
Michael Juen
Christoph Kreutz
Kathrin Breuker
spellingShingle Eva-Maria Schneeberger
Matthias Halper
Michael Palasser
Sarah Viola Heel
Jovana Vušurović
Raphael Plangger
Michael Juen
Christoph Kreutz
Kathrin Breuker
Native mass spectrometry reveals the initial binding events of HIV-1 rev to RRE stem II RNA
Nature Communications
author_facet Eva-Maria Schneeberger
Matthias Halper
Michael Palasser
Sarah Viola Heel
Jovana Vušurović
Raphael Plangger
Michael Juen
Christoph Kreutz
Kathrin Breuker
author_sort Eva-Maria Schneeberger
title Native mass spectrometry reveals the initial binding events of HIV-1 rev to RRE stem II RNA
title_short Native mass spectrometry reveals the initial binding events of HIV-1 rev to RRE stem II RNA
title_full Native mass spectrometry reveals the initial binding events of HIV-1 rev to RRE stem II RNA
title_fullStr Native mass spectrometry reveals the initial binding events of HIV-1 rev to RRE stem II RNA
title_full_unstemmed Native mass spectrometry reveals the initial binding events of HIV-1 rev to RRE stem II RNA
title_sort native mass spectrometry reveals the initial binding events of hiv-1 rev to rre stem ii rna
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-11-01
description The HIV-1 RNA-binding protein rev facilitates nuclear export of viral RNA. Here, the authors use native mass spectrometry to study the interactions between rev-derived peptides and rev response elements of HIV-1 RNA, providing mechanistic insights into rev recognition and recruitment.
url https://doi.org/10.1038/s41467-020-19144-7
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AT matthiashalper nativemassspectrometryrevealstheinitialbindingeventsofhiv1revtorrestemiirna
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AT christophkreutz nativemassspectrometryrevealstheinitialbindingeventsofhiv1revtorrestemiirna
AT kathrinbreuker nativemassspectrometryrevealstheinitialbindingeventsofhiv1revtorrestemiirna
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