Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.

We have used restriction isotyping (restriction enzyme isoform genotyping) for rapid typing of common apolipoprotein E isoforms (E2, E3, E4). ApoE restriction isotyping used oligonucleotides to amplify apolipoprotein E gene sequences containing amino acid positions 112 and 158. The amplification pro...

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Main Authors:	JE Hixson, DT Vernier
Format:	Article
Language:	English
Published:	Elsevier 1990-03-01
Series:	Journal of Lipid Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520431761

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spelling	doaj-13d412f6c2c04fff9a562d8b66f734702021-04-25T04:23:04ZengElsevierJournal of Lipid Research0022-22751990-03-01313545548Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.JE Hixson0DT Vernier1Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78284.Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78284.We have used restriction isotyping (restriction enzyme isoform genotyping) for rapid typing of common apolipoprotein E isoforms (E2, E3, E4). ApoE restriction isotyping used oligonucleotides to amplify apolipoprotein E gene sequences containing amino acid positions 112 and 158. The amplification products were digested with HhaI and subjected to electrophoresis on polyacrylamide gels. Each of the isoforms was distinguished by a unique combination of HhaI fragment sizes that enabled unambiguous typing of all homozygotic and heterozygotic combinations. HhaI cleaves at GCGC encoding 112arg (E4) and 158arg (E3, E4), but does not cut at GTGC encoding 112cys (E2, E3) and 158cys (E2).http://www.sciencedirect.com/science/article/pii/S0022227520431761
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	JE Hixson DT Vernier
spellingShingle	JE Hixson DT Vernier Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI. Journal of Lipid Research
author_facet	JE Hixson DT Vernier
author_sort	JE Hixson
title	Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.
title_short	Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.
title_full	Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.
title_fullStr	Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.
title_full_unstemmed	Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.
title_sort	restriction isotyping of human apolipoprotein e by gene amplification and cleavage with hhai.
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1990-03-01
description	We have used restriction isotyping (restriction enzyme isoform genotyping) for rapid typing of common apolipoprotein E isoforms (E2, E3, E4). ApoE restriction isotyping used oligonucleotides to amplify apolipoprotein E gene sequences containing amino acid positions 112 and 158. The amplification products were digested with HhaI and subjected to electrophoresis on polyacrylamide gels. Each of the isoforms was distinguished by a unique combination of HhaI fragment sizes that enabled unambiguous typing of all homozygotic and heterozygotic combinations. HhaI cleaves at GCGC encoding 112arg (E4) and 158arg (E3, E4), but does not cut at GTGC encoding 112cys (E2, E3) and 158cys (E2).
url	http://www.sciencedirect.com/science/article/pii/S0022227520431761
work_keys_str_mv	AT jehixson restrictionisotypingofhumanapolipoproteinebygeneamplificationandcleavagewithhhai AT dtvernier restrictionisotypingofhumanapolipoproteinebygeneamplificationandcleavagewithhhai
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Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.

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