Responses of Leaf Anatomy and CO2 Concentrating Mechanisms of the Aquatic Plant Ottelia cordata to Variable CO2
Acclimation to variable CO2 was studied in floating leaves of the freshwater monocot Ottelia cordata grown in either low or high CO2. The most striking anatomical variations responding to high CO2 included the enlarged upper epidermal cells and the decreased area of epidermal chloroplasts. Stomata t...
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doaj-138a9a4b029c4c90a4c268bb0fc474102020-11-25T03:37:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-08-011110.3389/fpls.2020.01261558999Responses of Leaf Anatomy and CO2 Concentrating Mechanisms of the Aquatic Plant Ottelia cordata to Variable CO2Wenmin Huang0Wenmin Huang1Shijuan Han2Shijuan Han3Zhenfei Xing4Wei Li5Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Center of Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan, ChinaAix Marseille Univ CNRS, BIP UMR 7281, IMM, FR 3479, Marseille, FranceKey Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Center of Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaKey Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Center of Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan, ChinaAcclimation to variable CO2 was studied in floating leaves of the freshwater monocot Ottelia cordata grown in either low or high CO2. The most striking anatomical variations responding to high CO2 included the enlarged upper epidermal cells and the decreased area of epidermal chloroplasts. Stomata that distributed on the upper surface, and the stomatic chamber area, showed no significant response to high CO2. pH-drift experiments indicated that floating leaves of O. cordata were able to use bicarbonate regardless of CO2 concentrations. Photosynthetic enzyme activities and patterns of organic acids fluctuation confirmed that floating leaves of O. cordata can operate CAM only at low CO2, and perform C4-like metabolism at both high and low CO2. Overall, the present results imply that the floating leaves of O. cordata does not just rely on the atmospheric CO2 for its inorganic carbon, but is also dependent on CO2 and bicarbonate in the water. By showing these effects of CO2 variation, we highlight the need for further experimental studies on the regulatory mechanisms in O. cordata floating leaves, that prevent futile cycling among the three CO2 concentrating mechanisms (bicarbonate use, C4, and CAM metabolism) and the strategy for exploiting atmospheric CO2, as well as studies on the detailed biochemical pathway for C4 and CAM metabolism in this species.https://www.frontiersin.org/article/10.3389/fpls.2020.01261/fullCO2 availabilityC4crassulacean acid metabolismbicarbonate useCO2 concentrating mechanismsorganic acids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenmin Huang Wenmin Huang Shijuan Han Shijuan Han Zhenfei Xing Wei Li |
spellingShingle |
Wenmin Huang Wenmin Huang Shijuan Han Shijuan Han Zhenfei Xing Wei Li Responses of Leaf Anatomy and CO2 Concentrating Mechanisms of the Aquatic Plant Ottelia cordata to Variable CO2 Frontiers in Plant Science CO2 availability C4 crassulacean acid metabolism bicarbonate use CO2 concentrating mechanisms organic acids |
author_facet |
Wenmin Huang Wenmin Huang Shijuan Han Shijuan Han Zhenfei Xing Wei Li |
author_sort |
Wenmin Huang |
title |
Responses of Leaf Anatomy and CO2 Concentrating Mechanisms of the Aquatic Plant Ottelia cordata to Variable CO2 |
title_short |
Responses of Leaf Anatomy and CO2 Concentrating Mechanisms of the Aquatic Plant Ottelia cordata to Variable CO2 |
title_full |
Responses of Leaf Anatomy and CO2 Concentrating Mechanisms of the Aquatic Plant Ottelia cordata to Variable CO2 |
title_fullStr |
Responses of Leaf Anatomy and CO2 Concentrating Mechanisms of the Aquatic Plant Ottelia cordata to Variable CO2 |
title_full_unstemmed |
Responses of Leaf Anatomy and CO2 Concentrating Mechanisms of the Aquatic Plant Ottelia cordata to Variable CO2 |
title_sort |
responses of leaf anatomy and co2 concentrating mechanisms of the aquatic plant ottelia cordata to variable co2 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2020-08-01 |
description |
Acclimation to variable CO2 was studied in floating leaves of the freshwater monocot Ottelia cordata grown in either low or high CO2. The most striking anatomical variations responding to high CO2 included the enlarged upper epidermal cells and the decreased area of epidermal chloroplasts. Stomata that distributed on the upper surface, and the stomatic chamber area, showed no significant response to high CO2. pH-drift experiments indicated that floating leaves of O. cordata were able to use bicarbonate regardless of CO2 concentrations. Photosynthetic enzyme activities and patterns of organic acids fluctuation confirmed that floating leaves of O. cordata can operate CAM only at low CO2, and perform C4-like metabolism at both high and low CO2. Overall, the present results imply that the floating leaves of O. cordata does not just rely on the atmospheric CO2 for its inorganic carbon, but is also dependent on CO2 and bicarbonate in the water. By showing these effects of CO2 variation, we highlight the need for further experimental studies on the regulatory mechanisms in O. cordata floating leaves, that prevent futile cycling among the three CO2 concentrating mechanisms (bicarbonate use, C4, and CAM metabolism) and the strategy for exploiting atmospheric CO2, as well as studies on the detailed biochemical pathway for C4 and CAM metabolism in this species. |
topic |
CO2 availability C4 crassulacean acid metabolism bicarbonate use CO2 concentrating mechanisms organic acids |
url |
https://www.frontiersin.org/article/10.3389/fpls.2020.01261/full |
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