At-Line Reversed Phase Liquid Chromatography for In-Process Monitoring of Inclusion Body Solubilization

• Main Authors: Julian Ebner, Diana Humer, Robert Klausser, Viktor Rubus, Reinhard Pell, Oliver Spadiut, Julian Kopp
• Format: Article
• Language: English
• Published: MDPI AG 2021-06-01
• Series: Bioengineering
• Subjects: inclusion bodies; inclusion body solubilization; tailored refolding; reversed phase liquid chromatography; process analytical technology tools; in-process monitoring
• Online Access: https://www.mdpi.com/2306-5354/8/6/78

Refolding is known as the bottleneck in inclusion body (IB) downstream processing in the pharmaceutical industry: high dilutions leading to large operating volumes, slow refolding kinetics and low refolding yields are only a few of the problems that impede industrial application. Solubilization prior to refolding is often carried out empirically and the effects of the solubilizate on the subsequent refolding step are rarely investigated. The results obtained in this study, however, indicate that the quality of the IB solubilizate has a severe effect on subsequent refolding. As the solubilizate contains chaotropic reagents in high molarities, it is commonly analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE, however, suffers from a long analysis time, making at-line analytical implementation difficult. In this study, we established an at-line reversed phase liquid chromatography method to investigate the time-dependent quality of the solubilizate. To verify the necessity of at-line solubilization monitoring, we varied the essential solubilization conditions for horseradish peroxidase IBs. The solubilization time was found to have a major influence on subsequent refolding, underlining the high need for an at-line analysis of solubilization. Furthermore, we used the developed reversed phase liquid chromatography method for an in-process control (IPC). In conclusion, the presented reversed phase liquid chromatography method allows a proper control of IB solubilization applicable for tailored refolding.