Sequence and phylogenetic analysis of novel porcine parvovirus 7 isolates from pigs in Guangxi, China.

Parvoviruses are a diverse group of viruses that infect a wide range of animals and humans. In recent years, advances in molecular techniques have resulted in the identification of several novel parvoviruses in swine. In this study, porcine parvovirus 7 (PPV7) isolates from clinical samples collecte...

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Main Authors: Wei Wang, Liang Cao, Wenchao Sun, Jialiang Xin, Min Zheng, Mingyao Tian, Huijun Lu, Ningyi Jin
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0219560
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spelling doaj-136faffcf00542e8ab068e13c84866362021-03-03T20:34:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01147e021956010.1371/journal.pone.0219560Sequence and phylogenetic analysis of novel porcine parvovirus 7 isolates from pigs in Guangxi, China.Wei WangLiang CaoWenchao SunJialiang XinMin ZhengMingyao TianHuijun LuNingyi JinParvoviruses are a diverse group of viruses that infect a wide range of animals and humans. In recent years, advances in molecular techniques have resulted in the identification of several novel parvoviruses in swine. In this study, porcine parvovirus 7 (PPV7) isolates from clinical samples collected in Guangxi, China, were examined to understand their molecular epidemiology and co-infection with porcine circovirus type 2 (PCV2). In this study, among the 385 pig serum samples, 105 were positive for PPV7, representing a 27.3% positive detection rate. The co-infection rate of PPV7 and PCV2 was 17.4% (67/385). Compared with the reference strains, we noted 93.9%-97.9% similarity in the NS1 gene and 87.4%-95.0% similarity in the cap gene. Interestingly, compared with the reference strains, sixteen of the PPV7 strains in this study contained an additional 3 to 15 nucleotides in the middle of the cap gene. Therefore, the Cap protein of fourteen strains encoded 474 amino acids, and the Cap protein of the other two strains encoded 470 amino acids. However, the Cap protein of the reference strain PPV7 isolate 42 encodes 469 amino acids. This is the first report of sequence variation within the cap gene, confirming an increase in the number of amino acids in the Cap protein of PPV7. Our findings provide new insight into the prevalence of PPV7 in swine in Guangxi, China, as well as sequence data and phylogenetic analysis of these novel PPV7 isolates.https://doi.org/10.1371/journal.pone.0219560
collection DOAJ
language English
format Article
sources DOAJ
author Wei Wang
Liang Cao
Wenchao Sun
Jialiang Xin
Min Zheng
Mingyao Tian
Huijun Lu
Ningyi Jin
spellingShingle Wei Wang
Liang Cao
Wenchao Sun
Jialiang Xin
Min Zheng
Mingyao Tian
Huijun Lu
Ningyi Jin
Sequence and phylogenetic analysis of novel porcine parvovirus 7 isolates from pigs in Guangxi, China.
PLoS ONE
author_facet Wei Wang
Liang Cao
Wenchao Sun
Jialiang Xin
Min Zheng
Mingyao Tian
Huijun Lu
Ningyi Jin
author_sort Wei Wang
title Sequence and phylogenetic analysis of novel porcine parvovirus 7 isolates from pigs in Guangxi, China.
title_short Sequence and phylogenetic analysis of novel porcine parvovirus 7 isolates from pigs in Guangxi, China.
title_full Sequence and phylogenetic analysis of novel porcine parvovirus 7 isolates from pigs in Guangxi, China.
title_fullStr Sequence and phylogenetic analysis of novel porcine parvovirus 7 isolates from pigs in Guangxi, China.
title_full_unstemmed Sequence and phylogenetic analysis of novel porcine parvovirus 7 isolates from pigs in Guangxi, China.
title_sort sequence and phylogenetic analysis of novel porcine parvovirus 7 isolates from pigs in guangxi, china.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2019-01-01
description Parvoviruses are a diverse group of viruses that infect a wide range of animals and humans. In recent years, advances in molecular techniques have resulted in the identification of several novel parvoviruses in swine. In this study, porcine parvovirus 7 (PPV7) isolates from clinical samples collected in Guangxi, China, were examined to understand their molecular epidemiology and co-infection with porcine circovirus type 2 (PCV2). In this study, among the 385 pig serum samples, 105 were positive for PPV7, representing a 27.3% positive detection rate. The co-infection rate of PPV7 and PCV2 was 17.4% (67/385). Compared with the reference strains, we noted 93.9%-97.9% similarity in the NS1 gene and 87.4%-95.0% similarity in the cap gene. Interestingly, compared with the reference strains, sixteen of the PPV7 strains in this study contained an additional 3 to 15 nucleotides in the middle of the cap gene. Therefore, the Cap protein of fourteen strains encoded 474 amino acids, and the Cap protein of the other two strains encoded 470 amino acids. However, the Cap protein of the reference strain PPV7 isolate 42 encodes 469 amino acids. This is the first report of sequence variation within the cap gene, confirming an increase in the number of amino acids in the Cap protein of PPV7. Our findings provide new insight into the prevalence of PPV7 in swine in Guangxi, China, as well as sequence data and phylogenetic analysis of these novel PPV7 isolates.
url https://doi.org/10.1371/journal.pone.0219560
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