Comparative study of commercially available and homemade anti-VAMP7 antibodies using CRISPR/Cas9-depleted HeLa cells and VAMP7 knockout mice [version 2; referees: 2 approved]

VAMP7 (vesicle-associated membrane protein) belongs to the intracellular membrane fusion SNARE (Soluble N-ethylmaleimide-sensitive factor attachment protein receptors) protein family. In this study, we used CRISPR/Cas9 genome editing technology to generate VAMP7 knockout (KO) human HeLa cells and mo...

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Bibliographic Details
Main Authors: Agathe Verraes, Beatrice Cholley, Thierry Galli, Sebastien Nola
Format: Article
Language:English
Published: F1000 Research Ltd 2019-02-01
Series:F1000Research
Online Access:https://f1000research.com/articles/7-1649/v2