Genetic Diversity in Commercial Rapeseed (Brassica napus L.) Varieties from Turkey as Revealed by RAPD

• Main Authors: Özlem ÖZBEK, Betül Uçar GIDIK
• Format: Article
• Language: English
• Published: University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca 2013-02-01
• Series: Notulae Scientia Biologicae
• Online Access: http://notulaebiologicae.ro/index.php/nsb/article/view/8911
• ISSN: 2067-3205; 2067-3264

<span style="font-family: Times New Roman; font-size: small;"> </span><p style="margin: 0in 0in 0pt; text-align: justify; text-indent: 0.5in;" class="MsoNormal"><span style="color: #221e1f; font-size: 10pt;"><span style="font-family: Times New Roman;">In cultivated commercial crop species, genetic diversity tends to decrease because of the extensive breeding processes. Therefore, germplasm of commercial crop species, such as </span></span><span style="color: #221e1f; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">Brassica napus </span><span style="color: #221e1f; font-size: 10pt;"><span style="font-family: Times New Roman;">L. should be evaluated and the genotypes, which have higher genetic diversity index, should be addressed as potential parental cross materials in breeding programs. In this study, the genetic diversity was analysed by using randomly amplified polymorphic DNA analysis (RAPD) technique in nine Turkish commercial rapeseed varieties. The RAPD primers (10-mer oligonucleotides) produced 51 scorable loci, 31 loci of which were polymorphic (60.78%) and 20 loci (39.22%) were monomorphic The RAPD bands were scored as binary matrix data and were analysed using POPGENE version 1.32. At locus level, the values of genetic diversity within population (</span></span><span style="color: #221e1f; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">H</span><span style="color: #221e1f;"><span class="A6"><span style="font-size: 5.5pt;"><em><span style="font-family: Garamond Premr Pro;">s</span></em></span></span><span style="color: #221e1f; font-size: 10pt;"><span style="font-family: Times New Roman;">) and total (</span></span><span style="color: #221e1f; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">H</span><span class="A6"><span style="font-size: 5.5pt;"><em><span style="font-family: Garamond Premr Pro;">T</span></em></span></span><span style="color: #221e1f; font-size: 10pt;"><span style="font-family: Times New Roman;">) were 0.15 and 0.19 respectively. The genetic differentiation (</span></span><span style="color: #221e1f; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">G</span><span class="A6"><span style="font-size: 5.5pt;"><em><span style="font-family: Garamond Premr Pro;">ST</span></em></span></span><span style="color: #221e1f; font-size: 10pt;"><span style="font-family: Times New Roman;">) and the gene flow (</span></span><span style="color: #221e1f; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">N</span><span class="A6"><span style="font-size: 5.5pt;"><em><span style="font-family: Garamond Premr Pro;">m</span></em></span></span><span style="color: #221e1f; font-size: 10pt;"><span style="font-family: Times New Roman;">) values between the populations were 0.20 and 2.05 respectively. The mean number of alleles (</span></span><span style="color: #221e1f; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">n</span><span class="A6"><span style="font-size: 5.5pt;"><em><span style="font-family: Garamond Premr Pro;">a</span></em></span></span><span style="color: #221e1f; font-size: 10pt;"><span style="font-family: Times New Roman;">), the mean number of effective alleles (</span></span><span style="color: #221e1f; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">n</span><span class="A6"><span style="font-size: 5.5pt;"><em><span style="font-family: Garamond Premr Pro;">ae</span></em></span></span><span style="color: #221e1f; font-size: 10pt;"><span style="font-family: Times New Roman;">), and the mean value of genetic diversity (</span></span><span style="color: #221e1f; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">H</span><span class="A6"><span style="font-size: 5.5pt;"><em><span style="font-family: Garamond Premr Pro;">e</span></em></span></span><span style="color: #221e1f; font-size: 10pt;"><span style="font-family: Times New Roman;">) were 2.00, 1.26, and 0.19 respectively. According to Pearson’s correlation, multiple regression and principal component analyses, eco-geographical conditions in combination had significant effect on genetic indices of commercial </span></span></span><span style="color: #221e1f; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">B. napus </span><span style="font-family: Times New Roman;"><span style="color: #221e1f; font-size: 10pt;">L. varieties were discussed.</span></span></p><span style="font-family: Times New Roman; font-size: small;"> </span>