Directed evolution of CRISPR-Cas9 to increase its specificity
Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity.
Bibliographic Details
| Main Authors: |
Jungjoon K. Lee,
Euihwan Jeong,
Joonsun Lee,
Minhee Jung,
Eunji Shin,
Young-hoon Kim,
Kangin Lee,
Inyoung Jung,
Daesik Kim,
Seokjoong Kim,
Jin-Soo Kim |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Publishing Group
2018-08-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/s41467-018-05477-x
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