Mycelium Polysaccharides from <i>Termitomyces albuminosus</i> Attenuate CCl<sub>4</sub>-Induced Chronic Liver Injury Via Inhibiting TGFβ1/Smad3 and NF-κB Signal Pathways

Mycelium Polysaccharides from <i>Termitomyces albuminosus</i> Attenuate CCl<sub>4</sub>-Induced Chronic Liver Injury Via Inhibiting TGFβ1/Smad3 and NF-κB Signal Pathways

A major fraction (MPT-W), eluted by deionized water, was extracted from mycelium polysaccharides of <i>Termitomyces albuminosus</i> (MPT), and its antioxidant, anti-fibrosis, and anti-inflammatory activities in CCl<sub>4</sub>-induced chronic liver injury mice, as well as pre...

Full description

Bibliographic Details
Main Authors: Huajie Zhao, Huaping Li, Yanbo Feng, Yiwen Zhang, Fangfang Yuan, Jianjun Zhang, Haixia Ren, Le Jia
Format: Article
Language:English
Published: MDPI AG 2019-09-01
Series:International Journal of Molecular Sciences
Subjects:
anti-oxidation
anti-inflammatory
anti-fibrosis
liver injury
mycelium polysaccharides
<i>termitomyces albuminosus</i>
Online Access:https://www.mdpi.com/1422-0067/20/19/4872
id doaj-12e16cd99bce4054b647aa00a8842f89
record_format Article
spelling doaj-12e16cd99bce4054b647aa00a8842f892020-11-25T01:41:44ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-09-012019487210.3390/ijms20194872ijms20194872Mycelium Polysaccharides from <i>Termitomyces albuminosus</i> Attenuate CCl<sub>4</sub>-Induced Chronic Liver Injury Via Inhibiting TGFβ1/Smad3 and NF-κB Signal PathwaysHuajie Zhao0Huaping Li1Yanbo Feng2Yiwen Zhang3Fangfang Yuan4Jianjun Zhang5Haixia Ren6Le Jia7Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Science, Jinan 250100, ChinaCollege of Life Science, Shandong Agricultural University, Taian 271018, ChinaCollege of Life Science, Shandong Agricultural University, Taian 271018, ChinaCollege of Life Science, Shandong Agricultural University, Taian 271018, ChinaCollege of Life Science, Shandong Agricultural University, Taian 271018, ChinaCollege of Life Science, Shandong Agricultural University, Taian 271018, ChinaInstitute of Agricultural Resources and Environment, Shandong Academy of Agricultural Science, Jinan 250100, ChinaCollege of Life Science, Shandong Agricultural University, Taian 271018, ChinaA major fraction (MPT-W), eluted by deionized water, was extracted from mycelium polysaccharides of <i>Termitomyces albuminosus</i> (MPT), and its antioxidant, anti-fibrosis, and anti-inflammatory activities in CCl<sub>4</sub>-induced chronic liver injury mice, as well as preliminary characterizations, were evaluated. The results showed that MPT-W was a polysaccharide of &#945;- and &#946;-configurations containing xylose (Xyl), fucose (Fuc), mannose (Man), galactose (Gal), and glucose (Glc) with a molar ratio of 0.29:8.67:37.89:35.98:16.60 by gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared (FT-IR) spectroscopy. Its molecular weight (Mw), obtained by high-performance gel permeation chromatography (HPGPC), was 1.30 &#215; 10<sup>5</sup> Da. The antioxidant assays in vitro showed that MPT-W displayed scavenging free-radical abilities. Based on the data of in vivo experiments, MPT-W could inhibit TGF&#946;1/Smad3 and NF-&#954;B pathways; decrease the level and activity of cytochrome P4502E1 (CYP2E1), malonaldehyde (MDA) and serum enzyme; activate the HO-1/Nrf2 pathway; and increase antioxidant enzymes to protect the liver in CCl<sub>4</sub>-induced chronic liver injury mice. Therefore, MPT-W could be a potentially natural and functional resource contributing to antioxidant, hepatoprotective, and anti-inflammatory effects with potential health benefits.https://www.mdpi.com/1422-0067/20/19/4872anti-oxidationanti-inflammatoryanti-fibrosisliver injurymycelium polysaccharides<i>termitomyces albuminosus</i>
collection DOAJ
language English
format Article
sources DOAJ
author Huajie Zhao
Huaping Li
Yanbo Feng
Yiwen Zhang
Fangfang Yuan
Jianjun Zhang
Haixia Ren
Le Jia
spellingShingle Huajie Zhao
Huaping Li
Yanbo Feng
Yiwen Zhang
Fangfang Yuan
Jianjun Zhang
Haixia Ren
Le Jia
Mycelium Polysaccharides from <i>Termitomyces albuminosus</i> Attenuate CCl<sub>4</sub>-Induced Chronic Liver Injury Via Inhibiting TGFβ1/Smad3 and NF-κB Signal Pathways
International Journal of Molecular Sciences
anti-oxidation
anti-inflammatory
anti-fibrosis
liver injury
mycelium polysaccharides
<i>termitomyces albuminosus</i>
author_facet Huajie Zhao
Huaping Li
Yanbo Feng
Yiwen Zhang
Fangfang Yuan
Jianjun Zhang
Haixia Ren
Le Jia
author_sort Huajie Zhao
title Mycelium Polysaccharides from <i>Termitomyces albuminosus</i> Attenuate CCl<sub>4</sub>-Induced Chronic Liver Injury Via Inhibiting TGFβ1/Smad3 and NF-κB Signal Pathways
title_short Mycelium Polysaccharides from <i>Termitomyces albuminosus</i> Attenuate CCl<sub>4</sub>-Induced Chronic Liver Injury Via Inhibiting TGFβ1/Smad3 and NF-κB Signal Pathways
title_full Mycelium Polysaccharides from <i>Termitomyces albuminosus</i> Attenuate CCl<sub>4</sub>-Induced Chronic Liver Injury Via Inhibiting TGFβ1/Smad3 and NF-κB Signal Pathways
title_fullStr Mycelium Polysaccharides from <i>Termitomyces albuminosus</i> Attenuate CCl<sub>4</sub>-Induced Chronic Liver Injury Via Inhibiting TGFβ1/Smad3 and NF-κB Signal Pathways
title_full_unstemmed Mycelium Polysaccharides from <i>Termitomyces albuminosus</i> Attenuate CCl<sub>4</sub>-Induced Chronic Liver Injury Via Inhibiting TGFβ1/Smad3 and NF-κB Signal Pathways
title_sort mycelium polysaccharides from <i>termitomyces albuminosus</i> attenuate ccl<sub>4</sub>-induced chronic liver injury via inhibiting tgfβ1/smad3 and nf-κb signal pathways
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2019-09-01
description A major fraction (MPT-W), eluted by deionized water, was extracted from mycelium polysaccharides of <i>Termitomyces albuminosus</i> (MPT), and its antioxidant, anti-fibrosis, and anti-inflammatory activities in CCl<sub>4</sub>-induced chronic liver injury mice, as well as preliminary characterizations, were evaluated. The results showed that MPT-W was a polysaccharide of &#945;- and &#946;-configurations containing xylose (Xyl), fucose (Fuc), mannose (Man), galactose (Gal), and glucose (Glc) with a molar ratio of 0.29:8.67:37.89:35.98:16.60 by gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared (FT-IR) spectroscopy. Its molecular weight (Mw), obtained by high-performance gel permeation chromatography (HPGPC), was 1.30 &#215; 10<sup>5</sup> Da. The antioxidant assays in vitro showed that MPT-W displayed scavenging free-radical abilities. Based on the data of in vivo experiments, MPT-W could inhibit TGF&#946;1/Smad3 and NF-&#954;B pathways; decrease the level and activity of cytochrome P4502E1 (CYP2E1), malonaldehyde (MDA) and serum enzyme; activate the HO-1/Nrf2 pathway; and increase antioxidant enzymes to protect the liver in CCl<sub>4</sub>-induced chronic liver injury mice. Therefore, MPT-W could be a potentially natural and functional resource contributing to antioxidant, hepatoprotective, and anti-inflammatory effects with potential health benefits.
topic anti-oxidation
anti-inflammatory
anti-fibrosis
liver injury
mycelium polysaccharides
<i>termitomyces albuminosus</i>
url https://www.mdpi.com/1422-0067/20/19/4872
work_keys_str_mv AT huajiezhao myceliumpolysaccharidesfromitermitomycesalbuminosusiattenuatecclsub4subinducedchronicliverinjuryviainhibitingtgfb1smad3andnfkbsignalpathways
AT huapingli myceliumpolysaccharidesfromitermitomycesalbuminosusiattenuatecclsub4subinducedchronicliverinjuryviainhibitingtgfb1smad3andnfkbsignalpathways
AT yanbofeng myceliumpolysaccharidesfromitermitomycesalbuminosusiattenuatecclsub4subinducedchronicliverinjuryviainhibitingtgfb1smad3andnfkbsignalpathways
AT yiwenzhang myceliumpolysaccharidesfromitermitomycesalbuminosusiattenuatecclsub4subinducedchronicliverinjuryviainhibitingtgfb1smad3andnfkbsignalpathways
AT fangfangyuan myceliumpolysaccharidesfromitermitomycesalbuminosusiattenuatecclsub4subinducedchronicliverinjuryviainhibitingtgfb1smad3andnfkbsignalpathways
AT jianjunzhang myceliumpolysaccharidesfromitermitomycesalbuminosusiattenuatecclsub4subinducedchronicliverinjuryviainhibitingtgfb1smad3andnfkbsignalpathways
AT haixiaren myceliumpolysaccharidesfromitermitomycesalbuminosusiattenuatecclsub4subinducedchronicliverinjuryviainhibitingtgfb1smad3andnfkbsignalpathways
AT lejia myceliumpolysaccharidesfromitermitomycesalbuminosusiattenuatecclsub4subinducedchronicliverinjuryviainhibitingtgfb1smad3andnfkbsignalpathways
_version_ 1725039945183133696

Similar Items