Genome-wide mapping of DNA double-strand breaks from eukaryotic cell cultures using Break-seq

Genome-wide mapping of DNA double-strand breaks from eukaryotic cell cultures using Break-seq

Summary: We describe a genome-wide DNA double-strand break (DSB) mapping technique, Break-seq. In this protocol, we provide step-by-step instructions for cell embedment in agarose, in-gel DSB labeling and subsequent capture, followed by standard Illumina library construction and sequencing. We also...

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Bibliographic Details
Main Authors: Ishita Joshi, Jenna DeRycke, Megan Palmowski, Robert LeSuer, Wenyi Feng
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Genomics
Sequencing
Model Organisms
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002616

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