Inhibitors of nuclear factor kappa B cause apoptosis in cultured macrophages
The precise role of the transcription factor nuclear factor kappa B (NF- κB) in the regulation of cell survival and cell death is still unresolved and may depend on cell type and position in the cell cycle. The aim of this study was to determine if three pharmacologic inhibitors of NF-κB, pyrrolidin...
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| Language: | English |
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Hindawi Limited
1997-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629359791721 |
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doaj-12c7abb9a50f491dab2a1b3a4cbde0cf2020-11-24T22:39:15ZengHindawi LimitedMediators of Inflammation0962-93511466-18611997-01-016322523210.1080/09629359791721Inhibitors of nuclear factor kappa B cause apoptosis in cultured macrophagesE. E. Mannick0J. Mishra1J. Marque2M. Clavell3M. J. S. Miller4P. D. Oliver5Department of Pediatrics, Louisiana State University, New Orleans, LA 70112, USADepartment of Pediatrics, Louisiana State University, New Orleans, LA 70112, USADepartment of Pediatrics, Louisiana State University, New Orleans, LA 70112, USADepartment of Pediatrics, Louisiana State University, New Orleans, LA 70112, USADepartment of Pediatrics, Louisiana State University, New Orleans, LA 70112, USADepartment of Pediatrics, Louisiana State University, New Orleans, LA 70112, USAThe precise role of the transcription factor nuclear factor kappa B (NF- κB) in the regulation of cell survival and cell death is still unresolved and may depend on cell type and position in the cell cycle. The aim of this study was to determine if three pharmacologic inhibitors of NF-κB, pyrrolidine dithiocarbamate, N-tosyl-L-lysl chloromethyl ketone and calpain I inhibitor, induce apoptosis in a murine macrophage cell line (RAW 264.7) at doses similar to those required for NF-κB inhibition. We found that each of the three inhibitors resulted in a dose- and time-dependent increase in morphologic indices of apoptosis in unstimulated, LPS-stimulated and TNF-stimulated cells. Lethal doses were consistent with those required for NF- κB inhibition. We conclude that nuclear NF-κB activation may represent an important survival mechanism in macrophages.http://dx.doi.org/10.1080/09629359791721 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
E. E. Mannick J. Mishra J. Marque M. Clavell M. J. S. Miller P. D. Oliver |
| spellingShingle |
E. E. Mannick J. Mishra J. Marque M. Clavell M. J. S. Miller P. D. Oliver Inhibitors of nuclear factor kappa B cause apoptosis in cultured macrophages Mediators of Inflammation |
| author_facet |
E. E. Mannick J. Mishra J. Marque M. Clavell M. J. S. Miller P. D. Oliver |
| author_sort |
E. E. Mannick |
| title |
Inhibitors of nuclear factor kappa B cause apoptosis in cultured macrophages |
| title_short |
Inhibitors of nuclear factor kappa B cause apoptosis in cultured macrophages |
| title_full |
Inhibitors of nuclear factor kappa B cause apoptosis in cultured macrophages |
| title_fullStr |
Inhibitors of nuclear factor kappa B cause apoptosis in cultured macrophages |
| title_full_unstemmed |
Inhibitors of nuclear factor kappa B cause apoptosis in cultured macrophages |
| title_sort |
inhibitors of nuclear factor kappa b cause apoptosis in cultured macrophages |
| publisher |
Hindawi Limited |
| series |
Mediators of Inflammation |
| issn |
0962-9351 1466-1861 |
| publishDate |
1997-01-01 |
| description |
The precise role of the transcription factor nuclear factor kappa B (NF- κB) in the regulation of cell survival and cell death is still unresolved and may depend on cell type and position in the cell cycle. The aim of this study was to determine if three pharmacologic inhibitors of NF-κB, pyrrolidine dithiocarbamate, N-tosyl-L-lysl chloromethyl ketone and calpain I inhibitor, induce apoptosis in a murine macrophage cell line (RAW 264.7) at doses similar to those required for NF-κB inhibition. We found that each of the three inhibitors resulted in a dose- and time-dependent increase in morphologic indices of apoptosis in unstimulated, LPS-stimulated and TNF-stimulated cells. Lethal doses were consistent with those required for NF- κB inhibition. We conclude that nuclear NF-κB activation may represent an important survival mechanism in macrophages. |
| url |
http://dx.doi.org/10.1080/09629359791721 |
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