Determination of Patulin By TLC From Mouldy Apples

• Main Author: Omar J. Al saree
• Format: Article
• Language: English
• Published: University of Baghdad, College of Veterinary Medicine 2010-06-01
• Series: The Iraqi Journal of Veterinary Medicine
• Online Access: https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/680

Analysis of the decayed apples (van. Starking delicious), collected from the local market, for patulin mycotoxin, using column chromotography and TLC Plates showed that the presence of the amount of patulin mycotoxin in the mouldy tissues (1.3 , 2.5 , 3.4 , 4.2, 5.5, and 6.4 ) cm in diameters were (5.1, 5.8, 6.3, 10.5, 12.3, and 14 ) μg patulin/g moldy tissues respectively. According to this research also, Patulin mycotoxin could diffused to the surrounding mouldy tissues. It is found that (0.5and 0.2), (1.0 and 0.5), and (1.5 and 0.5) μg patulin/g tissues in the first and second centimeters after mouldy tissues 4.2, 5.5 and 6.4 cm respectively, this phenomenon due to the presence of intracellular spaces in the tissues which allow to patulin to diffuse from mouldy to the healthy tissues there was no patulin found in the other surrounding healthy tissues. Gram positive bacteria found to be more susceptible to patulin, the zone of inhibition against Staphyllococcus aureus was 10 to 11 mm in diameter at concentration 10 and 30 mg /ml respectively when compared with 0.3 μg/ml streptomycin and 0.06 μg/ml ciprofloxacin while no inhibitory effect found against Gram negative bacteria Klebsiella pneumoniae and Escherichia coli