Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes

• Main Authors: Lívia Maria Mendonça Augusto, Diego Pinheiro Aguiar, Danielle Cabral Bonfim, Amanda dos Santos Cavalcanti, Priscila Ladeira Casado, Maria Eugênia Leite Duarte
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Ortopedia e Traumatologia 2016-02-01
• Series: Revista Brasileira de Ortopedia
• Subjects: Tendão; Células mesenquimais do estroma da medula óssea; Tenócitos
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162016000100070&lng=en&tlng=en

ABSTRACT OBJECTIVE: This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract. METHODS: Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR. RESULTS: The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression. CONCLUSION: Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes.