An <it>in silico</it> platform for the design of heterologous pathways in nonnative metabolite production

• Main Authors: Chatsurachai Sunisa, Furusawa Chikara, Shimizu Hiroshi
• Format: Article
• Language: English
• Published: BMC 2012-05-01
• Series: BMC Bioinformatics
• Online Access: http://www.biomedcentral.com/1471-2105/13/93

<p>Abstract</p> <p>Background</p> <p>Microorganisms are used as cell factories to produce valuable compounds in pharmaceuticals, biofuels, and other industrial processes. Incorporating heterologous metabolic pathways into well-characterized hosts is a major strategy for obtaining these target metabolites and improving productivity. However, selecting appropriate heterologous metabolic pathways for a host microorganism remains difficult owing to the complexity of metabolic networks. Hence, metabolic network design could benefit greatly from the availability of an <it>in silico</it> platform for heterologous pathway searching.</p> <p>Results</p> <p>We developed an algorithm for finding feasible heterologous pathways by which nonnative target metabolites are produced by host microorganisms, using <it>Escherichia coli</it>, <it>Corynebacterium glutamicum</it>, and <it>Saccharomyces cerevisiae</it> as templates. Using this algorithm, we screened heterologous pathways for the production of all possible nonnative target metabolites contained within databases. We then assessed the feasibility of the target productions using flux balance analysis, by which we could identify target metabolites associated with maximum cellular growth rate.</p> <p>Conclusions</p> <p>This <it>in silico</it> platform, designed for targeted searching of heterologous metabolic reactions, provides essential information for cell factory improvement.</p>