Optimal Thawing of Cryopreserved Peripheral Blood Mononuclear Cells for Use in High-Throughput Human Immune Monitoring Studies

Optimal Thawing of Cryopreserved Peripheral Blood Mononuclear Cells for Use in High-Throughput Human Immune Monitoring Studies

Cryopreserved peripheral blood mononuclear cells (PBMC) constitute an important component of immune monitoring studies as they allow for efficient batch- testing of samples as well as for the validation and extension of original studies in the future. In this study, we systematically test the permut...

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Bibliographic Details
Main Authors: Ramu A. Subbramanian, Paul V. Lehmann, Richard Caspell, Ioana Moldovan, Jessica Laux, Hari Ramachandran
Format: Article
Language:English
Published: MDPI AG 2012-07-01
Series:Cells
Subjects:
ELISPOT
PBMC
T cells
cryopreservation
DMSO
Online Access:http://www.mdpi.com/2073-4409/1/3/313

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