An assay suitable for high throughput screening of anti-influenza drugs.

An assay suitable for high throughput screening of anti-influenza drugs.

We developed a novel drug screening system for anti-influenza A virus by targeting the M2 proton channel. In the SPP (Single Protein Production) system, E. coli cell growth occurs only in the presence of effective M2 channel inhibitors, and thus simple measurement of cell growth was used as readouts...

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Bibliographic Details
Main Authors: Lili Mao, Jun Wang, William F DeGrado, Masayori Inouye
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3542334?pdf=render
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spelling doaj-125aba517baa46de84c7260393edd09e2020-11-25T01:00:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5407010.1371/journal.pone.0054070An assay suitable for high throughput screening of anti-influenza drugs.Lili MaoJun WangWilliam F DeGradoMasayori InouyeWe developed a novel drug screening system for anti-influenza A virus by targeting the M2 proton channel. In the SPP (Single Protein Production) system, E. coli cell growth occurs only in the presence of effective M2 channel inhibitors, and thus simple measurement of cell growth was used as readouts for drug screening. Two potential inhibitors for M2 (V27A) mutant were verified using this method, which inhibit both the mutant and wild-type M2 channels.http://europepmc.org/articles/PMC3542334?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Lili Mao
Jun Wang
William F DeGrado
Masayori Inouye
spellingShingle Lili Mao
Jun Wang
William F DeGrado
Masayori Inouye
An assay suitable for high throughput screening of anti-influenza drugs.
PLoS ONE
author_facet Lili Mao
Jun Wang
William F DeGrado
Masayori Inouye
author_sort Lili Mao
title An assay suitable for high throughput screening of anti-influenza drugs.
title_short An assay suitable for high throughput screening of anti-influenza drugs.
title_full An assay suitable for high throughput screening of anti-influenza drugs.
title_fullStr An assay suitable for high throughput screening of anti-influenza drugs.
title_full_unstemmed An assay suitable for high throughput screening of anti-influenza drugs.
title_sort assay suitable for high throughput screening of anti-influenza drugs.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description We developed a novel drug screening system for anti-influenza A virus by targeting the M2 proton channel. In the SPP (Single Protein Production) system, E. coli cell growth occurs only in the presence of effective M2 channel inhibitors, and thus simple measurement of cell growth was used as readouts for drug screening. Two potential inhibitors for M2 (V27A) mutant were verified using this method, which inhibit both the mutant and wild-type M2 channels.
url http://europepmc.org/articles/PMC3542334?pdf=render
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