Optimal use of statistical methods to validate reference gene stability in longitudinal studies.

Multiple statistical approaches have been proposed to validate reference genes in qPCR assays. However, conflicting results from these statistical methods pose a major hurdle in the choice of the best reference genes. Recent studies have proposed the use of at least three different methods but there...

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Bibliographic Details
Main Authors: Venkat Krishnan Sundaram, Nirmal Kumar Sampathkumar, Charbel Massaad, Julien Grenier
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0219440