The Search for Molecular Prognostic Markers of Diabetic Nephropathy in Patients with Type 2 Diabetes Mellitus

• Main Authors: V. M. Ibragimov, I.V. Sarvilina, M.M.Batiushin
• Format: Article
• Language: English
• Published: International Medical Research and Development Corporation 2016-03-01
• Series: International Journal of Biomedicine
• Subjects: type 2 diabetes mellitus; diabetic nephropathy; proteomics; molecular markers;
• Online Access: http://ijbm.org/articles/Article6(1)_OA14.pdf
• ISSN: 2158-0510; 2158-0529

The purpose of this study was to search for molecular prognostic markers of diabetic nephropathy (DN) in patients with type 2 diabetes mellitus (T2DM). The study included 205 patients with T2DM and DN (stages 1 to 4). All patients were stratified by the MDRD equation. The control group included 30 healthy individuals. All T2DM patients were divided into 4 groups depending on the DN stages. Group 1 included 42 patients with DN-Stage 1 (prenephropathy), Group 2 included 48 patients with DN-Stage 2 (incipient nephropathy); Group 3 included 65 patients with DN-Stage 3 (overt nephropathy), and Group 4 included 50 patients with DN-Stage 4 (kidney failure). Molecular phenotyping of urine was processed with methods of proteomics: the prefractionation, the separation of proteins with standard sets (MB-HIC C8 Kit, MB-IMAC Cu, MB-Wax Kit, «Bruker», USA), matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS, Ultraflex II, «Bruker», USA). The data of the molecular interactions and functional features of proteins were received with STRING 10.0 database. Potentially new molecular markers of DN development were identified. The research into signaling pathways and the molecules that are involved in ECM formation may help in developing strategies to prevent DN.