The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia

• Main Authors: Noha Mohamed El Husseiny, Walaa Abd El Fattah Attya Elkak, Mervat Said El Ansary, Engy Mohamed Abd El Aziz El Khateeb, Mervat Wagih Mattar, Doaa Mohamed El Demerdash
• Format: Article
• Language: English
• Published: Shiraz University of Medical Sciences 2017-03-01
• Series: Iranian Journal of Immunology
• Subjects: DCs Maturation; Monocyte-Derived DCs
• Online Access: http://iji.sums.ac.ir/article_39284_93c8bfa1ebac7e0690e7e6f4f911c298.pdf
• ISSN: 1735-1383; 1735-367X

<strong>Background:</strong> Generation of monocyte-derived dendritic cells (MDDC) is induced in the presence of GM-CSF and IL-4, and a maturation stimulus is added to the monocyte culture to obtain mature Dendritic Cells (DCs) suitable for therapy. TNF-α is the most common cytokine used for activating DCs and generating mature MDDC either alone or in combination with other cytokines. <strong>Objective:</strong> To compare effects of traditional cytokine cocktail (TNF-α + IL-1β) versus TLR4-agonist monophosphoryl lipid A on the viability, phenotype, cytokine profile and functionality of MDDC. <strong>Methods:</strong> The study included 32 individuals; twenty Acute Myeloid Leukaemia (AML) cases in complete remission and 12 healthy volunteers. They were divided into 3 groups: Group 1: control group: 12 subjects to measure the baseline levels of all markers in the monocytic preparation; Group 2: cytokine cocktail (TNF-α) group, which included 10 AML subjects; Group 3: MPLA group which included 10 AML subjects. <strong>Results:</strong> TNF-α group showed higher expression of CD83 than MPLA group indicating higher capacity to induce DC maturation but both were similar in CD86, CCR7 and IL-10 expression. Preparation of dendritic cells from AML cases in remission and loading them with tumor peptides was successful. <strong>Conclusion:</strong> The effect of MPLA in DC maturation is comparable with traditional DC maturation cocktail.