Biological Effects of Potato Plants Transformation with Glucose Oxidase Gene and their Resistance to Hyperthermia

• Main Authors: O.I. Grabelnych, O.A. Borovik, I.V. Lyubushkina, K.Z. Gamburg, A.V. Fedyaeva, I.V. Fedoseeva, A.V. Stepanov, E.G. Rikhvanov, D.V. Sauchyn, O.Yu. Urbanovich, G.B. Borovskii
• Format: Article
• Language: English
• Published: "Vikol publishing" ST Kolesnichenko V.V. 2017-02-01
• Series: Journal of Stress Physiology & Biochemistry
• Subjects: heat stress tolerance; high temperature; hydrogen peroxide; glucose oxidase; potato
• Online Access: http://www.jspb.ru/issues/2017/N1/JSPB_2017_1_05-14.pdf

It is known that regulation of plant tolerance to adverse environmental factors is connected with short term increase of the concentration of endogenous reactive oxygen species (ROS), which are signalling molecules for the induction of protective mechanisms. Introduction and expression of heterologous gox gene, which encodes glucose oxidase enzyme in plant genome, induce constantly higher content of hydrogen peroxide in plant tissues. It is not known how the introduction of native or modified gox gene affects the plant resistance to high-temperature stress, one of the most commonly used model for the study of stress response and thermal tolerance. In this study, we investigated biological effects of transformation and evaluated the resistance to temperature stress of potato plants with altered levels of glucose oxidase expression. Transformation of potato plants by gox gene led to the more early coming out from tuber dormancy of transformed plants and slower growth rate. Transformants containing the glucose oxidase gene were more sensitive to lethal thermal shock (50 °C, 90 min) than the transformant with the empty vector (pBI) or untransformed plants (CK). Pre-heating of plants at 37 °C significantly weakened the damaging effect of lethal thermal shock. This attenuation was more significant in the non-transformed plants.