Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol...

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Bibliographic Details
Main Authors: Jesse J. Waggoner, Victoria Stittleburg, Renee Pond, Youssef Saklawi, Malaya K. Sahoo, Ahmed Babiker, Laila Hussaini, Colleen S. Kraft, Benjamin A. Pinsky, Evan J. Anderson, Nadine Rouphael
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2020-07-01
Series:Emerging Infectious Diseases
Subjects:
COVID-19
SARS-CoV-2
rRT-PCR
molecular detection
respiratory infections
severe acute respiratory syndrome coronavirus 2
Online Access:https://wwwnc.cdc.gov/eid/article/26/7/20-1285_article
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spelling doaj-11e673c410d741968c11f4e65903d9202020-11-25T03:12:42ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592020-07-012671633163510.3201/eid2607.201285Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2Jesse J. WaggonerVictoria StittleburgRenee PondYoussef SaklawiMalaya K. SahooAhmed BabikerLaila HussainiColleen S. KraftBenjamin A. PinskyEvan J. AndersonNadine RouphaelMost reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.https://wwwnc.cdc.gov/eid/article/26/7/20-1285_articleCOVID-19SARS-CoV-2rRT-PCRmolecular detectionrespiratory infectionssevere acute respiratory syndrome coronavirus 2
collection DOAJ
language English
format Article
sources DOAJ
author Jesse J. Waggoner
Victoria Stittleburg
Renee Pond
Youssef Saklawi
Malaya K. Sahoo
Ahmed Babiker
Laila Hussaini
Colleen S. Kraft
Benjamin A. Pinsky
Evan J. Anderson
Nadine Rouphael
spellingShingle Jesse J. Waggoner
Victoria Stittleburg
Renee Pond
Youssef Saklawi
Malaya K. Sahoo
Ahmed Babiker
Laila Hussaini
Colleen S. Kraft
Benjamin A. Pinsky
Evan J. Anderson
Nadine Rouphael
Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
Emerging Infectious Diseases
COVID-19
SARS-CoV-2
rRT-PCR
molecular detection
respiratory infections
severe acute respiratory syndrome coronavirus 2
author_facet Jesse J. Waggoner
Victoria Stittleburg
Renee Pond
Youssef Saklawi
Malaya K. Sahoo
Ahmed Babiker
Laila Hussaini
Colleen S. Kraft
Benjamin A. Pinsky
Evan J. Anderson
Nadine Rouphael
author_sort Jesse J. Waggoner
title Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
title_short Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
title_full Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
title_fullStr Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
title_full_unstemmed Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
title_sort triplex real-time rt-pcr for severe acute respiratory syndrome coronavirus 2
publisher Centers for Disease Control and Prevention
series Emerging Infectious Diseases
issn 1080-6040
1080-6059
publishDate 2020-07-01
description Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
topic COVID-19
SARS-CoV-2
rRT-PCR
molecular detection
respiratory infections
severe acute respiratory syndrome coronavirus 2
url https://wwwnc.cdc.gov/eid/article/26/7/20-1285_article
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