In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in <i>Brassica juncea</i> and <i>Moringa oleifera</i>

The study aimed to investigate the antibacterial activity of Mustard (<i>Brassica juncea</i>) and Moringa (<i>Moringa oleifera</i>) leaf extracts and coagulant protein for their potential application in water treatment. Bacterial cell aggregation and growth kinetics studies w...

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Main Authors: Sangeeta Chandrashekar, Raman Vijayakumar, Ramachandran Chelliah, Eric Banan-Mwine Daliri, Inamul Hasan Madar, Ghazala Sultan, Momna Rubab, Fazle Elahi, Su-Jung Yeon, Deog-Hwan Oh
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/26/7/2080
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spelling doaj-11dd62788065434aa736fb9542d222152021-04-05T23:01:10ZengMDPI AGMolecules1420-30492021-04-01262080208010.3390/molecules26072080In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in <i>Brassica juncea</i> and <i>Moringa oleifera</i>Sangeeta Chandrashekar0Raman Vijayakumar1Ramachandran Chelliah2Eric Banan-Mwine Daliri3Inamul Hasan Madar4Ghazala Sultan5Momna Rubab6Fazle Elahi7Su-Jung Yeon8Deog-Hwan Oh9Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Physiology, Bharath Institute of Higher Education and Research, Chennai 600073, IndiaDepartment of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, IndiaDepartment of Computer Science, Aligarh Muslim University, Aligarh 202002, IndiaDepartment of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, KoreaThe study aimed to investigate the antibacterial activity of Mustard (<i>Brassica juncea</i>) and Moringa (<i>Moringa oleifera</i>) leaf extracts and coagulant protein for their potential application in water treatment. Bacterial cell aggregation and growth kinetics studies were employed for thirteen bacterial strains with different concentrations of leaf extracts and coagulant protein. <i>Moringa oleifera</i> leaf extract (MOS) and coagulant protein showed cell aggregation against ten bacterial strains, whereas leaf extract alone showed growth inhibition of five bacterial strains for up to 6 h and five bacterial strains for up to 3 h. <i>Brassica juncea</i> leaf extract (BJS) showed growth inhibition for up to 6 h, and three bacterial strains showed inhibition for up to 3 h. The highest inhibition concentration with 2.5 mg/mL was 19 mm, and furthermore, the minimum inhibitory concentration (MIC) (0.5 mg/mL) and MBC (1.5 mg/mL) were determined to have a higher antibacterial effect for <3 KDa peptides. Based on LCMS analysis, napin was identified in both MOS and BJS; furthermore, the mode of action of napin peptide was determined on lipoprotein X complex (LpxC) and four-chained structured binding protein of bacterial type II topoisomerase (4PLB). The docking analysis has exhibited moderate to potent inhibition with a range of dock score −912.9 Kcal/mol. Thus, it possesses antibacterial-coagulant potential bioactive peptides present in the <i>Moringa oleifera</i> purified protein (MOP) and <i>Brassica juncea</i> purified protein (BJP) that could act as an effective antimicrobial agent to replace currently available antibiotics. The result implies that MOP and <i>Brassica juncea</i> purified coagulant (BJP) proteins may perform a wide degree of antibacterial functions against different pathogens.https://www.mdpi.com/1420-3049/26/7/2080<i>Moringa oleifera</i><i>Brassica juncea</i>coagulant proteincell aggregationgrowth inhibitionLCMS
collection DOAJ
language English
format Article
sources DOAJ
author Sangeeta Chandrashekar
Raman Vijayakumar
Ramachandran Chelliah
Eric Banan-Mwine Daliri
Inamul Hasan Madar
Ghazala Sultan
Momna Rubab
Fazle Elahi
Su-Jung Yeon
Deog-Hwan Oh
spellingShingle Sangeeta Chandrashekar
Raman Vijayakumar
Ramachandran Chelliah
Eric Banan-Mwine Daliri
Inamul Hasan Madar
Ghazala Sultan
Momna Rubab
Fazle Elahi
Su-Jung Yeon
Deog-Hwan Oh
In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in <i>Brassica juncea</i> and <i>Moringa oleifera</i>
Molecules
<i>Moringa oleifera</i>
<i>Brassica juncea</i>
coagulant protein
cell aggregation
growth inhibition
LCMS
author_facet Sangeeta Chandrashekar
Raman Vijayakumar
Ramachandran Chelliah
Eric Banan-Mwine Daliri
Inamul Hasan Madar
Ghazala Sultan
Momna Rubab
Fazle Elahi
Su-Jung Yeon
Deog-Hwan Oh
author_sort Sangeeta Chandrashekar
title In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in <i>Brassica juncea</i> and <i>Moringa oleifera</i>
title_short In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in <i>Brassica juncea</i> and <i>Moringa oleifera</i>
title_full In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in <i>Brassica juncea</i> and <i>Moringa oleifera</i>
title_fullStr In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in <i>Brassica juncea</i> and <i>Moringa oleifera</i>
title_full_unstemmed In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in <i>Brassica juncea</i> and <i>Moringa oleifera</i>
title_sort in vitro and in silico screening and characterization of antimicrobial napin bioactive protein in <i>brassica juncea</i> and <i>moringa oleifera</i>
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2021-04-01
description The study aimed to investigate the antibacterial activity of Mustard (<i>Brassica juncea</i>) and Moringa (<i>Moringa oleifera</i>) leaf extracts and coagulant protein for their potential application in water treatment. Bacterial cell aggregation and growth kinetics studies were employed for thirteen bacterial strains with different concentrations of leaf extracts and coagulant protein. <i>Moringa oleifera</i> leaf extract (MOS) and coagulant protein showed cell aggregation against ten bacterial strains, whereas leaf extract alone showed growth inhibition of five bacterial strains for up to 6 h and five bacterial strains for up to 3 h. <i>Brassica juncea</i> leaf extract (BJS) showed growth inhibition for up to 6 h, and three bacterial strains showed inhibition for up to 3 h. The highest inhibition concentration with 2.5 mg/mL was 19 mm, and furthermore, the minimum inhibitory concentration (MIC) (0.5 mg/mL) and MBC (1.5 mg/mL) were determined to have a higher antibacterial effect for <3 KDa peptides. Based on LCMS analysis, napin was identified in both MOS and BJS; furthermore, the mode of action of napin peptide was determined on lipoprotein X complex (LpxC) and four-chained structured binding protein of bacterial type II topoisomerase (4PLB). The docking analysis has exhibited moderate to potent inhibition with a range of dock score −912.9 Kcal/mol. Thus, it possesses antibacterial-coagulant potential bioactive peptides present in the <i>Moringa oleifera</i> purified protein (MOP) and <i>Brassica juncea</i> purified protein (BJP) that could act as an effective antimicrobial agent to replace currently available antibiotics. The result implies that MOP and <i>Brassica juncea</i> purified coagulant (BJP) proteins may perform a wide degree of antibacterial functions against different pathogens.
topic <i>Moringa oleifera</i>
<i>Brassica juncea</i>
coagulant protein
cell aggregation
growth inhibition
LCMS
url https://www.mdpi.com/1420-3049/26/7/2080
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