Fertilitas Semen Beku dalam Tris Kuning Telur dan Skim yang Diberi Omega-3 pada Sapi Simmental dengan Ransum Berimbuhan Seng dan Selenium Minimal

This study aims to examine the quality of frozen semen in Tris egg yolk (TEY) extender and skimmed milk extender with or witout omega-3. A total of 18 Simmental bulls belong to Lembang Artificial Insemination Centre were divided into three groups. Each was fed with standard feed (R1), standard feeds...

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Main Authors: Asep Kurnia, Soeparna Soeparna, Raden Iis Arifiantini, Rahmat Hidayat
Format: Article
Language:English
Published: Universitas Udayana 2018-09-01
Series:Jurnal Veteriner
Online Access:https://ojs.unud.ac.id/index.php/jvet/article/view/32138
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spelling doaj-11ae735722a54a439021f5b97dc060852020-11-25T01:51:43ZengUniversitas UdayanaJurnal Veteriner1411-83272477-56652018-09-0119225126210.19087/jveteriner.2018.19.2.25132138Fertilitas Semen Beku dalam Tris Kuning Telur dan Skim yang Diberi Omega-3 pada Sapi Simmental dengan Ransum Berimbuhan Seng dan Selenium MinimalAsep Kurnia0Soeparna Soeparna1Raden Iis Arifiantini2Rahmat Hidayat3Mahasiswa Program Doktor, Bidang ilmu Peternakan, Fakultas Peternakan, Universitas Padjadjaran; Balai Inseminasi Buatan, Lembang, BandungLaboratorium Reproduksi Ternak dan Inseminasi Buatan, Fapet UnpadDivisi Reproduksi dan Kebidanan, Departemen Klinik, Reproduksi dan Kebidanan, Fakultas Kedokteran Hewan, Institut Pertanian BogorLaboratorium Nutrisi Ternak Ruminansia dan Kimia Makanan Ternak, Fapet Unpad Jln. Raya bandung Sumedang, Jatinangor, Sumedang, Jawa Barat, IndoneiaThis study aims to examine the quality of frozen semen in Tris egg yolk (TEY) extender and skimmed milk extender with or witout omega-3. A total of 18 Simmental bulls belong to Lembang Artificial Insemination Centre were divided into three groups. Each was fed with standard feed (R1), standard feeds supplemented with minimal Se and Zn (R2) and standard feed supplemented with maximal of Se and Zn concentration. Semen were collected using an artificial vagina and were evaluated macro- and microscopically. The semen then were divided into four tubes and each diluted with Skimmed, SkimmedOmega 3, TEY or TEY-O. The semen was then packed into a 0.25 ml straw and equilibrated at 5 oC for 4 hours, then frozen above liquid nitrogen vapor, and stored in liquid nitrogen container (-196 oC). The qualities of frozen semen were evaluated on the motility, individual score, viability and integrity of the plasma membrane of sperms. Sperm motility of bulls fed with standard feed (R1) in TEY extender and R3 in TEY and TEY-Omega-3 extender were higher (p <0.05) compared to the other combinations. No difference was found on the individual score. The viability of sperms in bulls fed with standard feed in SkimmedOmega-3 extender was higher than the other treatments and the highest sperm plasma membrane integrity was demonstrated by sperm in bull feeding with R2 in TEY extender.https://ojs.unud.ac.id/index.php/jvet/article/view/32138
collection DOAJ
language English
format Article
sources DOAJ
author Asep Kurnia
Soeparna Soeparna
Raden Iis Arifiantini
Rahmat Hidayat
spellingShingle Asep Kurnia
Soeparna Soeparna
Raden Iis Arifiantini
Rahmat Hidayat
Fertilitas Semen Beku dalam Tris Kuning Telur dan Skim yang Diberi Omega-3 pada Sapi Simmental dengan Ransum Berimbuhan Seng dan Selenium Minimal
Jurnal Veteriner
author_facet Asep Kurnia
Soeparna Soeparna
Raden Iis Arifiantini
Rahmat Hidayat
author_sort Asep Kurnia
title Fertilitas Semen Beku dalam Tris Kuning Telur dan Skim yang Diberi Omega-3 pada Sapi Simmental dengan Ransum Berimbuhan Seng dan Selenium Minimal
title_short Fertilitas Semen Beku dalam Tris Kuning Telur dan Skim yang Diberi Omega-3 pada Sapi Simmental dengan Ransum Berimbuhan Seng dan Selenium Minimal
title_full Fertilitas Semen Beku dalam Tris Kuning Telur dan Skim yang Diberi Omega-3 pada Sapi Simmental dengan Ransum Berimbuhan Seng dan Selenium Minimal
title_fullStr Fertilitas Semen Beku dalam Tris Kuning Telur dan Skim yang Diberi Omega-3 pada Sapi Simmental dengan Ransum Berimbuhan Seng dan Selenium Minimal
title_full_unstemmed Fertilitas Semen Beku dalam Tris Kuning Telur dan Skim yang Diberi Omega-3 pada Sapi Simmental dengan Ransum Berimbuhan Seng dan Selenium Minimal
title_sort fertilitas semen beku dalam tris kuning telur dan skim yang diberi omega-3 pada sapi simmental dengan ransum berimbuhan seng dan selenium minimal
publisher Universitas Udayana
series Jurnal Veteriner
issn 1411-8327
2477-5665
publishDate 2018-09-01
description This study aims to examine the quality of frozen semen in Tris egg yolk (TEY) extender and skimmed milk extender with or witout omega-3. A total of 18 Simmental bulls belong to Lembang Artificial Insemination Centre were divided into three groups. Each was fed with standard feed (R1), standard feeds supplemented with minimal Se and Zn (R2) and standard feed supplemented with maximal of Se and Zn concentration. Semen were collected using an artificial vagina and were evaluated macro- and microscopically. The semen then were divided into four tubes and each diluted with Skimmed, SkimmedOmega 3, TEY or TEY-O. The semen was then packed into a 0.25 ml straw and equilibrated at 5 oC for 4 hours, then frozen above liquid nitrogen vapor, and stored in liquid nitrogen container (-196 oC). The qualities of frozen semen were evaluated on the motility, individual score, viability and integrity of the plasma membrane of sperms. Sperm motility of bulls fed with standard feed (R1) in TEY extender and R3 in TEY and TEY-Omega-3 extender were higher (p <0.05) compared to the other combinations. No difference was found on the individual score. The viability of sperms in bulls fed with standard feed in SkimmedOmega-3 extender was higher than the other treatments and the highest sperm plasma membrane integrity was demonstrated by sperm in bull feeding with R2 in TEY extender.
url https://ojs.unud.ac.id/index.php/jvet/article/view/32138
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