Generation of an induced pluripotent stem cell line from a patient with retinitis pigmentosa caused by RP1 mutation

Generation of an induced pluripotent stem cell line from a patient with retinitis pigmentosa caused by RP1 mutation

We report the generation of the iPSC line LEIi005-B from a patient with retinitis pigmentosa caused by a dominant nonsense mutation in the RP1 gene (c.2098G>T p.E700X). Reprogramming of dermal fibroblasts was performed using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367...

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Main Authors: Xiao Zhang, Sang Yoon Moon, Dan Zhang, Shang-Chih Chen, Tina Lamey, Jennifer A. Thompson, Terri McLaren, John N. De Roach, Samuel McLenachan, Fred K. Chen
Format: Article
Language:English
Published: Elsevier 2019-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506119300820
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spelling doaj-1198ddccd6654d0d90bc83fe3d81499b2020-11-25T01:59:00ZengElsevierStem Cell Research1873-50612019-05-0137Generation of an induced pluripotent stem cell line from a patient with retinitis pigmentosa caused by RP1 mutationXiao Zhang0Sang Yoon Moon1Dan Zhang2Shang-Chih Chen3Tina Lamey4Jennifer A. Thompson5Terri McLaren6John N. De Roach7Samuel McLenachan8Fred K. Chen9Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Lions Eye Institute Australia, Nedlands, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Lions Eye Institute Australia, Nedlands, Western Australia, AustraliaLions Eye Institute Australia, Nedlands, Western Australia, AustraliaLions Eye Institute Australia, Nedlands, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaAustralian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Lions Eye Institute Australia, Nedlands, Western Australia, Australia; Corresponding author at: Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia.Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Lions Eye Institute Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, Australia; Department of Ophthalmology, Royal Perth Hospital, Perth, Western Australia, AustraliaWe report the generation of the iPSC line LEIi005-B from a patient with retinitis pigmentosa caused by a dominant nonsense mutation in the RP1 gene (c.2098G>T p.E700X). Reprogramming of dermal fibroblasts was performed using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53 to establish the clonal iPSC line LEIi005-B. LEIi005-B expressed pluripotent stem cell markers, had a normal karyotype and differentiated into endoderm, mesoderm and ectoderm.http://www.sciencedirect.com/science/article/pii/S1873506119300820
collection DOAJ
language English
format Article
sources DOAJ
author Xiao Zhang
Sang Yoon Moon
Dan Zhang
Shang-Chih Chen
Tina Lamey
Jennifer A. Thompson
Terri McLaren
John N. De Roach
Samuel McLenachan
Fred K. Chen
spellingShingle Xiao Zhang
Sang Yoon Moon
Dan Zhang
Shang-Chih Chen
Tina Lamey
Jennifer A. Thompson
Terri McLaren
John N. De Roach
Samuel McLenachan
Fred K. Chen
Generation of an induced pluripotent stem cell line from a patient with retinitis pigmentosa caused by RP1 mutation
Stem Cell Research
author_facet Xiao Zhang
Sang Yoon Moon
Dan Zhang
Shang-Chih Chen
Tina Lamey
Jennifer A. Thompson
Terri McLaren
John N. De Roach
Samuel McLenachan
Fred K. Chen
author_sort Xiao Zhang
title Generation of an induced pluripotent stem cell line from a patient with retinitis pigmentosa caused by RP1 mutation
title_short Generation of an induced pluripotent stem cell line from a patient with retinitis pigmentosa caused by RP1 mutation
title_full Generation of an induced pluripotent stem cell line from a patient with retinitis pigmentosa caused by RP1 mutation
title_fullStr Generation of an induced pluripotent stem cell line from a patient with retinitis pigmentosa caused by RP1 mutation
title_full_unstemmed Generation of an induced pluripotent stem cell line from a patient with retinitis pigmentosa caused by RP1 mutation
title_sort generation of an induced pluripotent stem cell line from a patient with retinitis pigmentosa caused by rp1 mutation
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2019-05-01
description We report the generation of the iPSC line LEIi005-B from a patient with retinitis pigmentosa caused by a dominant nonsense mutation in the RP1 gene (c.2098G>T p.E700X). Reprogramming of dermal fibroblasts was performed using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53 to establish the clonal iPSC line LEIi005-B. LEIi005-B expressed pluripotent stem cell markers, had a normal karyotype and differentiated into endoderm, mesoderm and ectoderm.
url http://www.sciencedirect.com/science/article/pii/S1873506119300820
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