Bio efficacy, and photocatalytic activity of the silver nanoparticles synthesized from Cryptolepis buchanani leaf extract

• Main Authors: Sudipta Panja, Anutosh Patra, Kalyani Khanra, Indranil Choudhuri, Bikash Pati, Nandan Bhattacharyya
• Format: Article
• Language: English
• Published: Iranian Society of Nanomedicine 2020-11-01
• Series: Nanomedicine Research Journal
• Subjects: cryptolepis buchanani; silver nanoparticle; green synthesis; cytotoxicity; photocatalytic activity
• Online Access: http://www.nanomedicine-rj.com/article_47991_a5b5210f2e1d111b862c55db5a57e179.pdf

The objective of the study is to synthesis silver nanoparticles from the leaf extract of Cryptolepis buchanani, a medically important plant,by green synthesis method. The synthesized nanoparticles have an average size of 17.05±5.27 nm, crystalline in nature with face cantered cubic structure, and positive surface charge. The nanoparticles are biologically active. It killed > 90 % of HeLa cells at 25 μg mL-1 concentration in-vitro cell cytotoxicity assay, with a LD50 value of 3.98 μg mL-1. The nanoparticles are less effective on MCF-7 and HEK-293 cell line, almost 70 % of MCF-7 populations were survived at highest concentration of 25 μg mL-1. In case of HEK-293 it killed almost 78 % of cells at the same concentration with a 9.45 μg mL-1 LD50 value. The synthesized nanoparticle lysed less than 2.5 % of red blood cells at 10 μg mL-1 concentration in-vitro. The nanoparticles degraded >90 % of methylene blue dye in presence of light in 8.5 h. These properties of the synthesized nanoparticles are unique, and make it promising for its future potential applications.