Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.

Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.

In studies where D-(-)-3-hydroxy[4,4,4-2H3]butyrate is employed as isotopic tracer in vivo, we have described a selected ion monitoring, gas-liquid chromatography-mass spectrometry micromethod which measures [2H3] tracer enrichment in 3-hydroxybutyrate and acetoacetate from 300-microliters blood sam...

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Main Authors: P F Bougneres, E O Balasse, P Ferré, D M Bier
Format: Article
Language:English
Published: Elsevier 1986-12-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520388520
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spelling doaj-118084e6aae44740a2a8fdd43f77dc802021-04-25T04:21:24ZengElsevierJournal of Lipid Research0022-22751986-12-01272215220Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.P F BougneresE O BalasseP FerréD M BierIn studies where D-(-)-3-hydroxy[4,4,4-2H3]butyrate is employed as isotopic tracer in vivo, we have described a selected ion monitoring, gas-liquid chromatography-mass spectrometry micromethod which measures [2H3] tracer enrichment in 3-hydroxybutyrate and acetoacetate from 300-microliters blood samples. For plasma samples in the physiologic range, intra- and interassay precisions for each ketone averaged better than +/- 1% and +/- 2%, respectively. The use of the method was validated by comparing kinetic data obtained with the above tracer with simultaneous flux data obtained with conventional D-(-)-3-hydroxy[3-14C]butyrate tracer in five fasted rats.http://www.sciencedirect.com/science/article/pii/S0022227520388520
collection DOAJ
language English
format Article
sources DOAJ
author P F Bougneres
E O Balasse
P Ferré
D M Bier
spellingShingle P F Bougneres
E O Balasse
P Ferré
D M Bier
Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.
Journal of Lipid Research
author_facet P F Bougneres
E O Balasse
P Ferré
D M Bier
author_sort P F Bougneres
title Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.
title_short Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.
title_full Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.
title_fullStr Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.
title_full_unstemmed Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.
title_sort determination of ketone body kinetics using a d-(-)-3-hydroxy[4,4,4-2h3]butyrate tracer.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1986-12-01
description In studies where D-(-)-3-hydroxy[4,4,4-2H3]butyrate is employed as isotopic tracer in vivo, we have described a selected ion monitoring, gas-liquid chromatography-mass spectrometry micromethod which measures [2H3] tracer enrichment in 3-hydroxybutyrate and acetoacetate from 300-microliters blood samples. For plasma samples in the physiologic range, intra- and interassay precisions for each ketone averaged better than +/- 1% and +/- 2%, respectively. The use of the method was validated by comparing kinetic data obtained with the above tracer with simultaneous flux data obtained with conventional D-(-)-3-hydroxy[3-14C]butyrate tracer in five fasted rats.
url http://www.sciencedirect.com/science/article/pii/S0022227520388520
work_keys_str_mv AT pfbougneres determinationofketonebodykineticsusingad3hydroxy4442h3butyratetracer
AT eobalasse determinationofketonebodykineticsusingad3hydroxy4442h3butyratetracer
AT pferre determinationofketonebodykineticsusingad3hydroxy4442h3butyratetracer
AT dmbier determinationofketonebodykineticsusingad3hydroxy4442h3butyratetracer
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