Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system

Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system

Human ELTD1 (Epidermal growth factor, latrophilin and seven-transmembrane domain-containing 1), an orphan G-protein-coupled receptor (GPCR) belonging to the adhesion GPCR family, has been reported as a novel regulator of angiogenesis and a potential anti-cancer therapeutic target. However, little is...

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Main Authors: Qian Luo, Cong Wei, Yan Long, Meng Zhang, Wei Shan, Honghu Li, Shuyang Cai, Yulin Xu, Pengxu Qian, He Huang
Format: Article
Language:English
Published: Elsevier 2021-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121001963
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record_format Article
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language English
format Article
sources DOAJ
author Qian Luo
Cong Wei
Yan Long
Meng Zhang
Wei Shan
Honghu Li
Shuyang Cai
Yulin Xu
Pengxu Qian
He Huang
spellingShingle Qian Luo
Cong Wei
Yan Long
Meng Zhang
Wei Shan
Honghu Li
Shuyang Cai
Yulin Xu
Pengxu Qian
He Huang
Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system
Stem Cell Research
author_facet Qian Luo
Cong Wei
Yan Long
Meng Zhang
Wei Shan
Honghu Li
Shuyang Cai
Yulin Xu
Pengxu Qian
He Huang
author_sort Qian Luo
title Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system
title_short Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system
title_full Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system
title_fullStr Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system
title_full_unstemmed Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system
title_sort generation of an eltd1 knockout human embryonic stem cell line by the icrispr/cas9 system
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-05-01
description Human ELTD1 (Epidermal growth factor, latrophilin and seven-transmembrane domain-containing 1), an orphan G-protein-coupled receptor (GPCR) belonging to the adhesion GPCR family, has been reported as a novel regulator of angiogenesis and a potential anti-cancer therapeutic target. However, little is known about the function of ELTD1, especially its undiscovered ligands. In this experiment, an ELTD1 homozygous knockout human embryonic stem cell line, FAHZUe001-A, was generated by the iCRISPR/Cas9 system to achieve a deeper understanding of ELTD1. The FAHZUe001-A was confirmed with normal karyotype, typical undifferentiated morphology, pluripotency and trilineage differentiation potential in vitro.
url http://www.sciencedirect.com/science/article/pii/S1873506121001963
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spelling doaj-117445a3ddfc4ed58f743a86635741022021-05-30T04:41:42ZengElsevierStem Cell Research1873-50612021-05-0153102350Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 systemQian Luo0Cong Wei1Yan Long2Meng Zhang3Wei Shan4Honghu Li5Shuyang Cai6Yulin Xu7Pengxu Qian8He Huang9BoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR ChinaBoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR ChinaBoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR ChinaBoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR ChinaBoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR ChinaBoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR ChinaBoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR ChinaBoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR ChinaBoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR China; Center of Stem Cell and Regenerative Medicine, School of Medicine, Zhejiang University, Hangzhou 310012, PR China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Corresponding authors at: BoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China.BoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR China; Center of Stem Cell and Regenerative Medicine, School of Medicine, Zhejiang University, Hangzhou 310012, PR China; Corresponding authors at: BoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China.Human ELTD1 (Epidermal growth factor, latrophilin and seven-transmembrane domain-containing 1), an orphan G-protein-coupled receptor (GPCR) belonging to the adhesion GPCR family, has been reported as a novel regulator of angiogenesis and a potential anti-cancer therapeutic target. However, little is known about the function of ELTD1, especially its undiscovered ligands. In this experiment, an ELTD1 homozygous knockout human embryonic stem cell line, FAHZUe001-A, was generated by the iCRISPR/Cas9 system to achieve a deeper understanding of ELTD1. The FAHZUe001-A was confirmed with normal karyotype, typical undifferentiated morphology, pluripotency and trilineage differentiation potential in vitro.http://www.sciencedirect.com/science/article/pii/S1873506121001963

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