Increased cell viability and proliferation in post-hypoxic hippocampal tissue culture treated with<em> Acalypha indica</em> root extract

• Main Authors: Sophie Yolanda, Endang W. Bachtiar, Nurhadi Ibrahim
• Format: Article
• Language: English
• Published: Faculty of Medicine Universitas Indonesia 2011-05-01
• Series: Medical Journal of Indonesia
• Online Access: http://mji.ui.ac.id/journal/index.php/mji/article/view/433

<p><strong>Background:</strong> This research was done to study the influence of <em>Acalypha indica</em> Linn root extract towards relative cell viability and proliferation as parameters of neurogenesis in post-hypoxic hippocampal tissue culture.</p><p><strong>Methods:</strong> Experimental<em> in vitro</em> study using 24 primary neuronal cell cultures obtained from adult <em>Sprague Dawley</em> rat exposed to hypoxia with 5% O₂/5% CO₂/N₂ balance gas for 24 hours. Post-hypoxia, <em>Acalypha indica</em> Linn root extract was added at doses of 10, 15, and 20 mg/mL to 3 treatment groups. No treatment was given to the control group. Each group consists of 6 samples. After 90 hours of incubation, relative cell viability was measured by using <em>3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide</em> (MTT) examination, and cell proliferation was measured by using <em>5-bromo2’-deoxy-uridine</em> (BrdU) for cell proliferation. Data was analyzed using one way ANOVA parametric tests, then further analyzed with post-hoc analysis.</p><p><strong>Results:</strong> The relative cell viability of rat hippocampal tissue culture treated with<em> Acalypha indica</em> Linn root extract with dose of 10, 15, and 20 mg/mL was significantly higher than control (176.95%, 220.62%, and 386.02% vs. 100%). Cell proliferation of rat hippocampal tissue culture treated with <em>Acalypha indica</em> Linn root extract with dose of 10, 15, and 20 mg/mL was significantly higher than control (0.132, 0.117, 0.114 vs 0.096).</p><p><strong>Conclusion:</strong><em> Acalypha indica</em> Linn root extract with doses of 10, 15, and 20 mg/mL can increase relative cell viability and proliferation in post-hypoxic hippocampal tissue culture. <em><strong>(Med J Indones 2011; 20:94-9)</strong></em></p><p><strong>Keywords:</strong><em> Acalypha indica Linn (akar kucing), cell proliferation, hypoxia, neurogenesis, relative cell viability</em></p>