The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis
The Salmonidae fish family is well represented in Romanian fauna, with a total of six species in the wild and reared in fish farms. Among them, the brown trout (Salmo trutta fario) can be found in all major Romanian river basins. However, anthropogenic activities might disrupt salmonids’ habitats, s...
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Agroprint Timisoara
2017-05-01
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doaj-10fd23eb5f2a461daa73d1d216926c612020-11-25T04:04:42ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762017-05-0150174781982The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysisRamona Nechifor0Gina-Oana Popa1Maria Samu2Marieta Costache3Doru Banaduc4Andreea Dudu5Sergiu Emil Georgescu6University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyLucian Blaga University of Sibiu, Faculty of Sciences, Applied Ecology Research CenterUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyThe Salmonidae fish family is well represented in Romanian fauna, with a total of six species in the wild and reared in fish farms. Among them, the brown trout (Salmo trutta fario) can be found in all major Romanian river basins. However, anthropogenic activities might disrupt salmonids’ habitats, so that inbreeding and genetic isolation might easily occur in the wild populations. We analyzed two wild brown trout populations from rivers targeted by anthropogenic activities, by using nuclear markers and genotyping in order to observe their genetic structure. We analyzed nine microsatellites and we observed their alleles frequencies, number of private alleles, observed and expected heterozygosity, as well as their population structure. The two populations are not in Hardy-Weinberg equilibrium for most of the loci and the inbreeding coefficient for both populations suggests a heterozygote deficit. Further sequencing data are needed in order to have a better view upon their complete genetic structure.http://spasb.ro/index.php/spasb/article/view/2290salmonidswild populationsmicrosatellitesgenotyping |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ramona Nechifor Gina-Oana Popa Maria Samu Marieta Costache Doru Banaduc Andreea Dudu Sergiu Emil Georgescu |
spellingShingle |
Ramona Nechifor Gina-Oana Popa Maria Samu Marieta Costache Doru Banaduc Andreea Dudu Sergiu Emil Georgescu The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis Scientific Papers Animal Science and Biotechnologies salmonids wild populations microsatellites genotyping |
author_facet |
Ramona Nechifor Gina-Oana Popa Maria Samu Marieta Costache Doru Banaduc Andreea Dudu Sergiu Emil Georgescu |
author_sort |
Ramona Nechifor |
title |
The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis |
title_short |
The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis |
title_full |
The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis |
title_fullStr |
The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis |
title_full_unstemmed |
The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis |
title_sort |
genetic profiles of two salmonid populations from romania obtained through nuclear markers analysis |
publisher |
Agroprint Timisoara |
series |
Scientific Papers Animal Science and Biotechnologies |
issn |
1841-9364 2344-4576 |
publishDate |
2017-05-01 |
description |
The Salmonidae fish family is well represented in Romanian fauna, with a total of six species in the wild and reared in fish farms. Among them, the brown trout (Salmo trutta fario) can be found in all major Romanian river basins. However, anthropogenic activities might disrupt salmonids’ habitats, so that inbreeding and genetic isolation might easily occur in the wild populations. We analyzed two wild brown trout populations from rivers targeted by anthropogenic activities, by using nuclear markers and genotyping in order to observe their genetic structure. We analyzed nine microsatellites and we observed their alleles frequencies, number of private alleles, observed and expected heterozygosity, as well as their population structure. The two populations are not in Hardy-Weinberg equilibrium for most of the loci and the inbreeding coefficient for both populations suggests a heterozygote deficit. Further sequencing data are needed in order to have a better view upon their complete genetic structure. |
topic |
salmonids wild populations microsatellites genotyping |
url |
http://spasb.ro/index.php/spasb/article/view/2290 |
work_keys_str_mv |
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