The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis

The Salmonidae fish family is well represented in Romanian fauna, with a total of six species in the wild and reared in fish farms. Among them, the brown trout (Salmo trutta fario) can be found in all major Romanian river basins. However, anthropogenic activities might disrupt salmonids’ habitats, s...

Full description

Bibliographic Details
Main Authors: Ramona Nechifor, Gina-Oana Popa, Maria Samu, Marieta Costache, Doru Banaduc, Andreea Dudu, Sergiu Emil Georgescu
Format: Article
Language:English
Published: Agroprint Timisoara 2017-05-01
Series:Scientific Papers Animal Science and Biotechnologies
Subjects:
Online Access:http://spasb.ro/index.php/spasb/article/view/2290
id doaj-10fd23eb5f2a461daa73d1d216926c61
record_format Article
spelling doaj-10fd23eb5f2a461daa73d1d216926c612020-11-25T04:04:42ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762017-05-0150174781982The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysisRamona Nechifor0Gina-Oana Popa1Maria Samu2Marieta Costache3Doru Banaduc4Andreea Dudu5Sergiu Emil Georgescu6University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyLucian Blaga University of Sibiu, Faculty of Sciences, Applied Ecology Research CenterUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular BiologyThe Salmonidae fish family is well represented in Romanian fauna, with a total of six species in the wild and reared in fish farms. Among them, the brown trout (Salmo trutta fario) can be found in all major Romanian river basins. However, anthropogenic activities might disrupt salmonids’ habitats, so that inbreeding and genetic isolation might easily occur in the wild populations. We analyzed two wild brown trout populations from rivers targeted by anthropogenic activities, by using nuclear markers and genotyping in order to observe their genetic structure. We analyzed nine microsatellites and we observed their alleles frequencies, number of private alleles, observed and expected heterozygosity, as well as their population structure. The two populations are not in Hardy-Weinberg equilibrium for most of the loci and the inbreeding coefficient for both populations suggests a heterozygote deficit. Further sequencing data are needed in order to have a better view upon their complete genetic structure.http://spasb.ro/index.php/spasb/article/view/2290salmonidswild populationsmicrosatellitesgenotyping
collection DOAJ
language English
format Article
sources DOAJ
author Ramona Nechifor
Gina-Oana Popa
Maria Samu
Marieta Costache
Doru Banaduc
Andreea Dudu
Sergiu Emil Georgescu
spellingShingle Ramona Nechifor
Gina-Oana Popa
Maria Samu
Marieta Costache
Doru Banaduc
Andreea Dudu
Sergiu Emil Georgescu
The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis
Scientific Papers Animal Science and Biotechnologies
salmonids
wild populations
microsatellites
genotyping
author_facet Ramona Nechifor
Gina-Oana Popa
Maria Samu
Marieta Costache
Doru Banaduc
Andreea Dudu
Sergiu Emil Georgescu
author_sort Ramona Nechifor
title The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis
title_short The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis
title_full The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis
title_fullStr The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis
title_full_unstemmed The genetic profiles of two salmonid populations from Romania obtained through nuclear markers analysis
title_sort genetic profiles of two salmonid populations from romania obtained through nuclear markers analysis
publisher Agroprint Timisoara
series Scientific Papers Animal Science and Biotechnologies
issn 1841-9364
2344-4576
publishDate 2017-05-01
description The Salmonidae fish family is well represented in Romanian fauna, with a total of six species in the wild and reared in fish farms. Among them, the brown trout (Salmo trutta fario) can be found in all major Romanian river basins. However, anthropogenic activities might disrupt salmonids’ habitats, so that inbreeding and genetic isolation might easily occur in the wild populations. We analyzed two wild brown trout populations from rivers targeted by anthropogenic activities, by using nuclear markers and genotyping in order to observe their genetic structure. We analyzed nine microsatellites and we observed their alleles frequencies, number of private alleles, observed and expected heterozygosity, as well as their population structure. The two populations are not in Hardy-Weinberg equilibrium for most of the loci and the inbreeding coefficient for both populations suggests a heterozygote deficit. Further sequencing data are needed in order to have a better view upon their complete genetic structure.
topic salmonids
wild populations
microsatellites
genotyping
url http://spasb.ro/index.php/spasb/article/view/2290
work_keys_str_mv AT ramonanechifor thegeneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT ginaoanapopa thegeneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT mariasamu thegeneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT marietacostache thegeneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT dorubanaduc thegeneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT andreeadudu thegeneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT sergiuemilgeorgescu thegeneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT ramonanechifor geneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT ginaoanapopa geneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT mariasamu geneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT marietacostache geneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT dorubanaduc geneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT andreeadudu geneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
AT sergiuemilgeorgescu geneticprofilesoftwosalmonidpopulationsfromromaniaobtainedthroughnuclearmarkersanalysis
_version_ 1724435673647153152