A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies

A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies

The <i>Agrobacterium rhizogenes</i> hairy root transformation system is widely used in symbiotic studies of model legumes. It typically relies on fluorescent reporters, such as DsRed, for identification of transgenic roots. The MtLAP1 transcription factor has been utilized as a reporter...

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Bibliographic Details
Main Authors: Yiting Ruan, Ke Chen, Yangyang Su, Suyu Jiang, Ping Xu, Jeremy D. Murray
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:Plants
Subjects:
screening marker
<i>MtLAP1</i>
tissue-specific expression
hairy root transformation
root cap
<i>M. truncatula</i>
Online Access:https://www.mdpi.com/2223-7747/10/3/605
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spelling doaj-10d1c2b62a824aa0a3da5ab654696afd2021-03-24T00:02:51ZengMDPI AGPlants2223-77472021-03-011060560510.3390/plants10030605A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic StudiesYiting Ruan0Ke Chen1Yangyang Su2Suyu Jiang3Ping Xu4Jeremy D. Murray5CAS-JIC Center of Excellence for Plant and Microbial Science (CEPAMS), Center for Excellence in Molecular Plant Sciences (CEMPS), Shanghai Institute of Plant Physiology and Ecology (SIPPE), Chinese Academy of Sciences, Shanghai 200030, ChinaShanghai Engineering Research Center of Plant Germplasm Resource, College of Life Sciences, Shanghai Normal University, Shanghai 200234, ChinaShanghai Engineering Research Center of Plant Germplasm Resource, College of Life Sciences, Shanghai Normal University, Shanghai 200234, ChinaCAS-JIC Center of Excellence for Plant and Microbial Science (CEPAMS), Center for Excellence in Molecular Plant Sciences (CEMPS), Shanghai Institute of Plant Physiology and Ecology (SIPPE), Chinese Academy of Sciences, Shanghai 200030, ChinaShanghai Engineering Research Center of Plant Germplasm Resource, College of Life Sciences, Shanghai Normal University, Shanghai 200234, ChinaCAS-JIC Center of Excellence for Plant and Microbial Science (CEPAMS), Center for Excellence in Molecular Plant Sciences (CEMPS), Shanghai Institute of Plant Physiology and Ecology (SIPPE), Chinese Academy of Sciences, Shanghai 200030, ChinaThe <i>Agrobacterium rhizogenes</i> hairy root transformation system is widely used in symbiotic studies of model legumes. It typically relies on fluorescent reporters, such as DsRed, for identification of transgenic roots. The MtLAP1 transcription factor has been utilized as a reporter system in <i>Medicago truncatula</i> based on production of anthocyanin pigment. Here, we describe a version of this reporter driven by a root-cap specific promoter for direct observation of anthocyanin accumulation in root tips, which allows the identification of transgenic hairy roots by the naked eye. Results from our analysis suggest that the reporter had no significant effects on nodulation of <i>M. truncatula</i>. This approach, by virtue of its strong and specific expression in root cap cells, greatly reduces false positives and false negatives, and its use of an easily scored visible pigment should allow greater versatility and efficiency in root biology studies.https://www.mdpi.com/2223-7747/10/3/605screening marker<i>MtLAP1</i>tissue-specific expressionhairy root transformationroot cap<i>M. truncatula</i>
collection DOAJ
language English
format Article
sources DOAJ
author Yiting Ruan
Ke Chen
Yangyang Su
Suyu Jiang
Ping Xu
Jeremy D. Murray
spellingShingle Yiting Ruan
Ke Chen
Yangyang Su
Suyu Jiang
Ping Xu
Jeremy D. Murray
A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies
Plants
screening marker
<i>MtLAP1</i>
tissue-specific expression
hairy root transformation
root cap
<i>M. truncatula</i>
author_facet Yiting Ruan
Ke Chen
Yangyang Su
Suyu Jiang
Ping Xu
Jeremy D. Murray
author_sort Yiting Ruan
title A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies
title_short A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies
title_full A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies
title_fullStr A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies
title_full_unstemmed A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies
title_sort root tip-specific expressing anthocyanin marker for direct identification of transgenic tissues by the naked eye in symbiotic studies
publisher MDPI AG
series Plants
issn 2223-7747
publishDate 2021-03-01
description The <i>Agrobacterium rhizogenes</i> hairy root transformation system is widely used in symbiotic studies of model legumes. It typically relies on fluorescent reporters, such as DsRed, for identification of transgenic roots. The MtLAP1 transcription factor has been utilized as a reporter system in <i>Medicago truncatula</i> based on production of anthocyanin pigment. Here, we describe a version of this reporter driven by a root-cap specific promoter for direct observation of anthocyanin accumulation in root tips, which allows the identification of transgenic hairy roots by the naked eye. Results from our analysis suggest that the reporter had no significant effects on nodulation of <i>M. truncatula</i>. This approach, by virtue of its strong and specific expression in root cap cells, greatly reduces false positives and false negatives, and its use of an easily scored visible pigment should allow greater versatility and efficiency in root biology studies.
topic screening marker
<i>MtLAP1</i>
tissue-specific expression
hairy root transformation
root cap
<i>M. truncatula</i>
url https://www.mdpi.com/2223-7747/10/3/605
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