Dry mass estimation of tropical aquatic insects using different short-term preservation methods

Relationships of body mass and head capsule width were calculated for Thraulodes sp., Haplohyphes sp. (Ephemeroptera), Leptonema sp. and Nectopsyche sp. (Trichoptera), and Anacroneuria sp. (Plecoptera) using different preservatives (Freezing, Formaldehyde 4% and Kahle). The organisms were collected...

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Bibliographic Details
Main Author: Claudia Cressa
Format: Article
Language:English
Published: Vicerractoría Investigación 1999-06-01
Series:Revista de Biología Tropical
Subjects:
Online Access:http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77441999000100016
Description
Summary:Relationships of body mass and head capsule width were calculated for Thraulodes sp., Haplohyphes sp. (Ephemeroptera), Leptonema sp. and Nectopsyche sp. (Trichoptera), and Anacroneuria sp. (Plecoptera) using different preservatives (Freezing, Formaldehyde 4% and Kahle). The organisms were collected monthly during a year on the Orituco river, Venezuela with a Surber net (0. 1296 m² and 0.286 mm mesh size). The data presented here are representative of the organism conditions year around. No attempt was made to quantify intersample variation. Regression analysis indicated that all relationships were highly correlated for any of the fixatives used. Changes in dry mass per unit change of head capsule width, vary among species and preservatives with no clear relationship among them. Changes in dry mass calculated as the difference between dry mass of preserved samples to those of unpreserved ones, indicate that all fixatives underestimate dry mass by as much as 85.4%, except for Nectopsyche sp. whose dry mass was always overestimated. These results provide further evidence on the effect of preservatives on dry mass losses. Even when working with tropical species, any study in which biomass is going to be determined should consider the effect of preservatives on dry mass.<br>Se calcularon las ecuaciones para las relaciones entre la masa del cuerpo y el ancho de la cápsula cefálica para Thraulodes sp., Haplohyphes sp. (Ephemeroptera), Leptonema sp., Nectopsyche sp. (Trichoptera) y Anacroneuria sp. (Plecoptera) usando diferentes preservativos (Congelamiento, Formol 4% and Kahle). El análisis de regresión indicó que todas las relaciones obtenidas son altamente significativas para cada uno de los preservativos utilizados. Los cambios en la masa del cuerpo por unidad de cambio en el ancho de la cápsula cefálica, son diferentes para cada especie y preservativo, sin haberse obtenido una clara relación entre ellos. La compararación entre los valores de masa obtenidos con los diferentes preservativos y los obtenidos con muestras no preservadas, indica que todos los preservativos subestiman la masa seca hasta en un 85,4%, excepto en el caso de Nectopsyche sp. cuya masa corporal fue siempre sobre-estimada. Los resultados presentados en este trabajo nuevamente indican, aún trabajando con insectos acuáticos tropicales, que cualquier estudio en el cual la biomasa va a ser determinada, debe de tener en cuenta el efecto que los preservativos producen en la disminución de la masa corporal.
ISSN:0034-7744
2215-2075