Prevention and Detection of Mycoplasma Contamination in Cell Culture

• Main Authors: Parvaneh Farzaneh, Laleh Nikfarjam
• Format: Article
• Language: English
• Published: Royan Institute (ACECR), Tehran 2012-01-01
• Series: Cell Journal
• Subjects: Mycoplasma; Cell Culture; Prevention; Elimination
• Online Access: http://celljournal.org/library/upload/article/af_2284335225237336223226646554262278225254gorji.pdf

One of the main problems in cell culture is mycoplasma infection. It can extensively affectcell physiology and metabolism. As the applications of cell culture increase in research,industrial production and cell therapy, more concerns about mycoplasma contaminationand detection will arise. This review will provide valuable information about: 1. the waysin which cells are contaminated and the frequency and source of mycoplasma species incell culture; 2. the ways to prevent mycoplasma contamination in cell culture; 3. the importanceof mycoplasma tests in cell culture; 4. different methods to identify mycoplasmacontamination; 5. the consequences of mycoplasma contamination in cell culture and 6.available methods to eliminate mycoplasma contamination. Awareness about the sourcesof mycoplasma and pursuing aseptic techniques in cell culture along with reliable detectionmethods of mycoplasma contamination can provide an appropriate situation to preventmycoplasma contamination in cell culture.