Down-expression of TaPIN1s Increases the Tiller Number and Grain Yield in Wheat

• Main Authors: Fu Quan Yao, Xiao Hui Li, He Wang, Yu Ning Song, Zhong Qing Li, Xing Guo Li, Xin-Qi Gao, Xian Sheng Zhang, Xiao Min Bie
• Format: Article
• Language: English
• Published: BMC 2021-09-01
• Series: BMC Plant Biology
• Subjects: TaPIN1 genes; Tiller number; Grain yield; Wheat
• Online Access: https://doi.org/10.1186/s12870-021-03217-w

Abstract Background Tiller number is a factor determining panicle number and grain yield in wheat (Triticum aestivum). Auxin plays an important role in the regulation of branch production. PIN-FORMED 1 (PIN1), an auxin efflux carrier, plays a role in the regulation of tiller number in rice (Oryza sativa); however, little is known on the roles of PIN1 in wheat. Results Nine homologs of TaPIN1 genes were identified in wheat, of which TaPIN1-6 genes showed higher expression in the stem apex and young leaf in wheat, and the TaPIN1-6a protein was localized in the plasma membrane. The down-expression of TaPIN1s increased the tiller number in TaPIN1-RNA interference (TaPIN1-RNAi) transgenic wheat plants, indicating that auxin might mediate the axillary bud production. By contrast, the spikelet number, grain number per panicle, and the 1000-grain weight were decreased in the TaPIN1-RNAi transgenic wheat plants compared with those in the wild type. In summary, a reduction of TaPIN1s expression increased the tiller number and grain yield per plant of wheat. Conclusions Phylogenetic analysis and protein structure of nine TaPIN1 proteins were analyzed, and subcellular localization of TaPIN1-6a was located in the plasma membrane. Knock-down expression of TaPIN1 genes increased the tiller number of transgenic wheat lines. Our study suggests that TaPIN1s is required for the regulation of grain yield in wheat.