| Summary: | The article presents the comparative analysis of osseointegrative properties of different nanostructured carbon (a-C and CN0.25) coatings of the needles which is based on a study of regulating bone remodeling growth factors as well as a marker of apoptosis and bone resorption TNF-related apoptosis-inducing ligand (TRAIL).
Material and Methods ― The experiment was performed on 64 male rats of the Wistar line. Animals were immobilized by resection of the hind limb bones in proximal part of the right calf in order to simulate immobilization osteoporosis (IOP). Needles with carbon nanostructured CN0.25-coatings were implanted for the rats of the first group (32 rats), needles with diamond-like nanostructured a-C were implanted for the rats of the second group (32 rats). On 30th, 60th and 90th day after implantation we determined the concentrations of bone morphogenetic protein 2 (BMP-2), transforming growth factor beta (TGF-ß), TRAIL and the bone matrix resorption marker RatLaps in rats serum by ELISA.
Results ― The level of osteogenic growth factors concentrations was higher in group with a-C-coatings in comparison to that in group with CN0.25-coated needles (BMP-2 on the 90th day, TGF-ß1 on the 60th and 90th days), probably because of more pronounced cells and intercellular matrix elements adhesion to a-C-coatings. CN0.25-coatings demonstrated more pronounced activation of the bone resorption factor: TRAIL – a marker of apoptosis and a cytokine that promotes the activation of resorptive cells osteoclasts was higher in all observed periods.
Conclusion ― Thus, the results of the study indicated that effect of nanostructured a-C and CN0.25 coatings with various surface topography and chemical composition on the process of bone remodeling was different.
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