Rhizobacteria Inoculation Effects on Phytohormone Status of Potato Microclones Cultivated In Vitro under Osmotic Stress

• Main Authors: Tatiana N. Arkhipova, Nina V. Evseeva, Oksana V. Tkachenko, Gennady L. Burygin, Lidiya B. Vysotskaya, Zarina A. Akhtyamova, Guzel R. Kudoyarova
• Format: Article
• Language: English
• Published: MDPI AG 2020-08-01
• Series: Biomolecules
• Subjects: Solanum tuberosum; in vitro culture; Azospirillum brasilense Sp245; Ochrobactrum cytisi IPA7.2; auxin; cytokinins
• Online Access: https://www.mdpi.com/2218-273X/10/9/1231

Water deficits inhibit plant growth and decrease crop productivity. Remedies are needed to counter this increasingly urgent problem in practical farming. One possible approach is to utilize rhizobacteria known to increase plant resistance to abiotic and other stresses. We therefore studied the effects of inoculating the culture medium of potato microplants grown in vitro with <i>Azospirillum brasilense</i> Sp245 or <i>Ochrobactrum cytisi</i> IPA7.2. Growth and hormone content of the plants were evaluated under stress-free conditions and under a water deficit imposed with polyethylene glycol (PEG 6000). Inoculation with either bacterium promoted the growth in terms of leaf mass accumulation. The effects were associated with increased concentrations of auxin and cytokinin hormones in the leaves and stems and with suppression of an increase in the leaf abscisic acid that PEG treatment otherwise promoted in the potato microplants. <i>O. cytisi</i> IPA7.2 had a greater growth-stimulating effect than <i>A. brasilense</i> Sp245 on stressed plants, while <i>A. brasilense</i> Sp245 was more effective in unstressed plants. The effects were likely to be the result of changes to the plant’s hormonal balance brought about by the bacteria.