Assay of L-Arginine in rat blood by enzymatic method
<p>The study of enzymatic-chemical method of L-Arginine (Arg) assay on the real samples of rat blood was done. The developed method is based on the usage of recombinant arginase I and 2,3-butandion monooxim. Urea, the final product of the reaction, is detected by fluorometry. The new method wa...
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doaj-10b087e64dae41d1af18684cf68ad9992020-11-25T00:19:02ZengPC Technology CenterScienceRise2313-62862313-84162015-11-01116 (16)333810.15587/2313-8416.2015.5448651778Assay of L-Arginine in rat blood by enzymatic methodГалина Зуфарівна Гайда0Наталія Євгенівна Стасюк1Наталія Олександрівна Cибірна2Михайло Васильович Гончар3Institute of Cell Biology NAS of Ukraine 14/16 Drahomanov str., Lviv, Ukraine, 79005 5Institute of Cell Biology NAS of Ukraine 14/16 Drahomanov str., Lviv, Ukraine, 79005I. Franko Lviv National University 1 Universytetska str., Lviv, Ukraine, 79000Institute of Cell Biology NAS of Ukraine 14/16 Drahomanov str., Lviv, Ukraine, 79005<p>The study of enzymatic-chemical method of L-Arginine (Arg) assay on the real samples of rat blood was done. The developed method is based on the usage of recombinant arginase I and 2,3-butandion monooxim. Urea, the final product of the reaction, is detected by fluorometry. The new method was shown to be suitable for Arg assay in biological liquids of mammalians<em></em></p>http://journals.uran.ua/sciencerise/article/view/54486Аrginineurearat bloodenzymatic-chemical assayarginase23-butandion monooxim |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Галина Зуфарівна Гайда Наталія Євгенівна Стасюк Наталія Олександрівна Cибірна Михайло Васильович Гончар |
spellingShingle |
Галина Зуфарівна Гайда Наталія Євгенівна Стасюк Наталія Олександрівна Cибірна Михайло Васильович Гончар Assay of L-Arginine in rat blood by enzymatic method ScienceRise Аrginine urea rat blood enzymatic-chemical assay arginase 2 3-butandion monooxim |
author_facet |
Галина Зуфарівна Гайда Наталія Євгенівна Стасюк Наталія Олександрівна Cибірна Михайло Васильович Гончар |
author_sort |
Галина Зуфарівна Гайда |
title |
Assay of L-Arginine in rat blood by enzymatic method |
title_short |
Assay of L-Arginine in rat blood by enzymatic method |
title_full |
Assay of L-Arginine in rat blood by enzymatic method |
title_fullStr |
Assay of L-Arginine in rat blood by enzymatic method |
title_full_unstemmed |
Assay of L-Arginine in rat blood by enzymatic method |
title_sort |
assay of l-arginine in rat blood by enzymatic method |
publisher |
PC Technology Center |
series |
ScienceRise |
issn |
2313-6286 2313-8416 |
publishDate |
2015-11-01 |
description |
<p>The study of enzymatic-chemical method of L-Arginine (Arg) assay on the real samples of rat blood was done. The developed method is based on the usage of recombinant arginase I and 2,3-butandion monooxim. Urea, the final product of the reaction, is detected by fluorometry. The new method was shown to be suitable for Arg assay in biological liquids of mammalians<em></em></p> |
topic |
Аrginine urea rat blood enzymatic-chemical assay arginase 2 3-butandion monooxim |
url |
http://journals.uran.ua/sciencerise/article/view/54486 |
work_keys_str_mv |
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