Assay of L-Arginine in rat blood by enzymatic method

<p>The study of enzymatic-chemical method of L-Arginine (Arg) assay on the real samples of rat blood was done. The developed method is based on the usage of recombinant arginase I and 2,3-butandion monooxim. Urea, the final product of the reaction, is detected by fluorometry. The new method wa...

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Main Authors: Галина Зуфарівна Гайда, Наталія Євгенівна Стасюк, Наталія Олександрівна Cибірна, Михайло Васильович Гончар
Format: Article
Language:English
Published: PC Technology Center 2015-11-01
Series:ScienceRise
Subjects:
2
Online Access:http://journals.uran.ua/sciencerise/article/view/54486
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spelling doaj-10b087e64dae41d1af18684cf68ad9992020-11-25T00:19:02ZengPC Technology CenterScienceRise2313-62862313-84162015-11-01116 (16)333810.15587/2313-8416.2015.5448651778Assay of L-Arginine in rat blood by enzymatic methodГалина Зуфарівна Гайда0Наталія Євгенівна Стасюк1Наталія Олександрівна Cибірна2Михайло Васильович Гончар3Institute of Cell Biology NAS of Ukraine 14/16 Drahomanov str., Lviv, Ukraine, 79005 5Institute of Cell Biology NAS of Ukraine 14/16 Drahomanov str., Lviv, Ukraine, 79005I. Franko Lviv National University 1 Universytetska str., Lviv, Ukraine, 79000Institute of Cell Biology NAS of Ukraine 14/16 Drahomanov str., Lviv, Ukraine, 79005<p>The study of enzymatic-chemical method of L-Arginine (Arg) assay on the real samples of rat blood was done. The developed method is based on the usage of recombinant arginase I and 2,3-butandion monooxim. Urea, the final product of the reaction, is detected by fluorometry. The new method was shown to be suitable for Arg assay in biological liquids of mammalians<em></em></p>http://journals.uran.ua/sciencerise/article/view/54486Аrginineurearat bloodenzymatic-chemical assayarginase23-butandion monooxim
collection DOAJ
language English
format Article
sources DOAJ
author Галина Зуфарівна Гайда
Наталія Євгенівна Стасюк
Наталія Олександрівна Cибірна
Михайло Васильович Гончар
spellingShingle Галина Зуфарівна Гайда
Наталія Євгенівна Стасюк
Наталія Олександрівна Cибірна
Михайло Васильович Гончар
Assay of L-Arginine in rat blood by enzymatic method
ScienceRise
Аrginine
urea
rat blood
enzymatic-chemical assay
arginase
2
3-butandion monooxim
author_facet Галина Зуфарівна Гайда
Наталія Євгенівна Стасюк
Наталія Олександрівна Cибірна
Михайло Васильович Гончар
author_sort Галина Зуфарівна Гайда
title Assay of L-Arginine in rat blood by enzymatic method
title_short Assay of L-Arginine in rat blood by enzymatic method
title_full Assay of L-Arginine in rat blood by enzymatic method
title_fullStr Assay of L-Arginine in rat blood by enzymatic method
title_full_unstemmed Assay of L-Arginine in rat blood by enzymatic method
title_sort assay of l-arginine in rat blood by enzymatic method
publisher PC Technology Center
series ScienceRise
issn 2313-6286
2313-8416
publishDate 2015-11-01
description <p>The study of enzymatic-chemical method of L-Arginine (Arg) assay on the real samples of rat blood was done. The developed method is based on the usage of recombinant arginase I and 2,3-butandion monooxim. Urea, the final product of the reaction, is detected by fluorometry. The new method was shown to be suitable for Arg assay in biological liquids of mammalians<em></em></p>
topic Аrginine
urea
rat blood
enzymatic-chemical assay
arginase
2
3-butandion monooxim
url http://journals.uran.ua/sciencerise/article/view/54486
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AT mihajlovasilʹovičgončar assayoflarginineinratbloodbyenzymaticmethod
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