Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium elaterium methanol fruits extract

Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium elaterium methanol fruits extract

Abstract Ecballium elaterium species are mostly used as therapeutic agents and food ingredient. The current work was designed to investigate phytochemical contents, antioxidant, antibacterial, and anti-inflammatory properties of methanol fruits extract of Ecballium elaterium. Good antioxidant activi...

Full description

Bibliographic Details
Main Authors: Samir FELHI, Mongi SAOUDI, Amal DAOUD, Hafedh HAJLAOUI, Marwa NCIR, Rim CHAABANE, Abdelfattah EL FEKI, Néji GHARSALLAH, Adel KADRI
Format: Article
Language:English
Published: Sociedade Brasileira de Ciência e Tecnologia de Alimentos
Series:Food Science and Technology
Subjects:
Ecballium elaterium methanol fruits extract
phytochemical contents
FT-IR analysis
in vitro antioxidant and antibacterial behavior
in vivo anti-inflammatory potential
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017005011101&lng=en&tlng=en
id doaj-10858d1f612a4a03bf6a5bb6e7a70f60
record_format Article
spelling doaj-10858d1f612a4a03bf6a5bb6e7a70f602020-11-24T23:23:20ZengSociedade Brasileira de Ciência e Tecnologia de AlimentosFood Science and Technology1678-457X010.1590/1678-457x.26316S0101-20612017005011101Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium elaterium methanol fruits extractSamir FELHIMongi SAOUDIAmal DAOUDHafedh HAJLAOUIMarwa NCIRRim CHAABANEAbdelfattah EL FEKINéji GHARSALLAHAdel KADRIAbstract Ecballium elaterium species are mostly used as therapeutic agents and food ingredient. The current work was designed to investigate phytochemical contents, antioxidant, antibacterial, and anti-inflammatory properties of methanol fruits extract of Ecballium elaterium. Good antioxidant activity was observed with IC50 values of 156 ± 4 and 377 ± 6 μg/mL for DPPH and ABTS, respectively, and EC50 of 126 ± 4 µg/mL for FRAP assays, which is related with their richness in total phenolic, flavonoid and condensed tannins contents. The results of antibacterial activity showed the effectiveness of methanol extract against Bacillus cereus with value of inhibition zone diameter of 15 ± 0 mm and a MIC and MBC values of 6 ± 0 and 12 ± 0 mg/mL, respectively. The in vivo anti-inflammatory effects have been also studied by carrageenan induced rat paw edema assay and the results revealed that a dose of 75 mg/kg induced a significant inhibition of 66.4% at 2 h. FT-IR spectral data justified the presence of biological functional groups such as ─OH, C─H, C─O, C─C and C=O. These results highlighted the potential using of Ecballium elaterium fruits extract as natural antimicrobial, antioxidant and anti-inflammatory agents for food applications and for the pharmaceutical industry.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017005011101&lng=en&tlng=enEcballium elaterium methanol fruits extractphytochemical contentsFT-IR analysisin vitro antioxidant and antibacterial behaviorin vivo anti-inflammatory potential
collection DOAJ
language English
format Article
sources DOAJ
author Samir FELHI
Mongi SAOUDI
Amal DAOUD
Hafedh HAJLAOUI
Marwa NCIR
Rim CHAABANE
Abdelfattah EL FEKI
Néji GHARSALLAH
Adel KADRI
spellingShingle Samir FELHI
Mongi SAOUDI
Amal DAOUD
Hafedh HAJLAOUI
Marwa NCIR
Rim CHAABANE
Abdelfattah EL FEKI
Néji GHARSALLAH
Adel KADRI
Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium elaterium methanol fruits extract
Food Science and Technology
Ecballium elaterium methanol fruits extract
phytochemical contents
FT-IR analysis
in vitro antioxidant and antibacterial behavior
in vivo anti-inflammatory potential
author_facet Samir FELHI
Mongi SAOUDI
Amal DAOUD
Hafedh HAJLAOUI
Marwa NCIR
Rim CHAABANE
Abdelfattah EL FEKI
Néji GHARSALLAH
Adel KADRI
author_sort Samir FELHI
title Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium elaterium methanol fruits extract
title_short Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium elaterium methanol fruits extract
title_full Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium elaterium methanol fruits extract
title_fullStr Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium elaterium methanol fruits extract
title_full_unstemmed Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium elaterium methanol fruits extract
title_sort investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of ecballium elaterium methanol fruits extract
publisher Sociedade Brasileira de Ciência e Tecnologia de Alimentos
series Food Science and Technology
issn 1678-457X
description Abstract Ecballium elaterium species are mostly used as therapeutic agents and food ingredient. The current work was designed to investigate phytochemical contents, antioxidant, antibacterial, and anti-inflammatory properties of methanol fruits extract of Ecballium elaterium. Good antioxidant activity was observed with IC50 values of 156 ± 4 and 377 ± 6 μg/mL for DPPH and ABTS, respectively, and EC50 of 126 ± 4 µg/mL for FRAP assays, which is related with their richness in total phenolic, flavonoid and condensed tannins contents. The results of antibacterial activity showed the effectiveness of methanol extract against Bacillus cereus with value of inhibition zone diameter of 15 ± 0 mm and a MIC and MBC values of 6 ± 0 and 12 ± 0 mg/mL, respectively. The in vivo anti-inflammatory effects have been also studied by carrageenan induced rat paw edema assay and the results revealed that a dose of 75 mg/kg induced a significant inhibition of 66.4% at 2 h. FT-IR spectral data justified the presence of biological functional groups such as ─OH, C─H, C─O, C─C and C=O. These results highlighted the potential using of Ecballium elaterium fruits extract as natural antimicrobial, antioxidant and anti-inflammatory agents for food applications and for the pharmaceutical industry.
topic Ecballium elaterium methanol fruits extract
phytochemical contents
FT-IR analysis
in vitro antioxidant and antibacterial behavior
in vivo anti-inflammatory potential
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017005011101&lng=en&tlng=en
work_keys_str_mv AT samirfelhi investigationofphytochemicalcontentsinvitroantioxidantandantibacterialbehaviorandinvivoantiinflammatorypotentialofecballiumelateriummethanolfruitsextract
AT mongisaoudi investigationofphytochemicalcontentsinvitroantioxidantandantibacterialbehaviorandinvivoantiinflammatorypotentialofecballiumelateriummethanolfruitsextract
AT amaldaoud investigationofphytochemicalcontentsinvitroantioxidantandantibacterialbehaviorandinvivoantiinflammatorypotentialofecballiumelateriummethanolfruitsextract
AT hafedhhajlaoui investigationofphytochemicalcontentsinvitroantioxidantandantibacterialbehaviorandinvivoantiinflammatorypotentialofecballiumelateriummethanolfruitsextract
AT marwancir investigationofphytochemicalcontentsinvitroantioxidantandantibacterialbehaviorandinvivoantiinflammatorypotentialofecballiumelateriummethanolfruitsextract
AT rimchaabane investigationofphytochemicalcontentsinvitroantioxidantandantibacterialbehaviorandinvivoantiinflammatorypotentialofecballiumelateriummethanolfruitsextract
AT abdelfattahelfeki investigationofphytochemicalcontentsinvitroantioxidantandantibacterialbehaviorandinvivoantiinflammatorypotentialofecballiumelateriummethanolfruitsextract
AT nejigharsallah investigationofphytochemicalcontentsinvitroantioxidantandantibacterialbehaviorandinvivoantiinflammatorypotentialofecballiumelateriummethanolfruitsextract
AT adelkadri investigationofphytochemicalcontentsinvitroantioxidantandantibacterialbehaviorandinvivoantiinflammatorypotentialofecballiumelateriummethanolfruitsextract
_version_ 1725564062088036352

Similar Items