Histological Examination of Callogenesis in Bisected Protocorm Culture of Pigeon Orchid (Dendrobium crumenatum Swartz)
Callogenesis is a prerequisite step for the initiation of callus-mediated plant regeneration. Three-month-old protocorms of Dendrobium crumenatum Swartz (pigeon orchid) were bisected transversely and cultured on modified Vacin and Went (VW) solid medium containing 20 g L-1 sucrose, 2 g L-1 Phytagel...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Walailak University
2015-06-01
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| Series: | Walailak Journal of Science and Technology |
| Subjects: | |
| Online Access: | http://wjst.wu.ac.th/index.php/wjst/article/view/1606 |
| Summary: | Callogenesis is a prerequisite step for the initiation of callus-mediated plant regeneration. Three-month-old protocorms of Dendrobium crumenatum Swartz (pigeon orchid) were bisected transversely and cultured on modified Vacin and Went (VW) solid medium containing 20 g L-1 sucrose, 2 g L-1 Phytagel added with various concentrations of 1-naphthaleneacetic acid (NAA) (0, 0.1, 0.5 mg L-1) and 6-benzyladenine (BA) (0, 1, 2 mg L-1). The highest percentage of callus formation was obtained from the explant cultured on a modified VW supplemented with 0.5 mg L-1 NAA and 1 mg L-1 BA. The callus originated from the subepidermal layer of the bisected protocorm and subsequently formed nodular structure after culture for 8 weeks. The callus mass contained small isodiametric cells with prominent nuclei and nucleoli within dense cytoplasm. |
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| ISSN: | 1686-3933 2228-835X |