sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine
Abstract We present a base editing system, in which base editors are attached to different sites of sgRNA scaffold (sgBE). Each independent sgBE has its own specific editing pattern for a given target site. Among tested sgBEs, sgBE-SL4, in which deaminase is attached to the last stem-loop of sgRNA,...
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BMC
2020-08-01
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| Series: | Genome Biology |
| Online Access: | http://link.springer.com/article/10.1186/s13059-020-02137-6 |
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doaj-1071e35ca00c42869521a0116b225db42020-11-25T03:43:33ZengBMCGenome Biology1474-760X2020-08-0121111610.1186/s13059-020-02137-6sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosineYanhong Wang0Lifang Zhou1Rui Tao2Nan Liu3Jie Long4Fengming Qin5Wenling Tang6Yang Yang7Qiang Chen8Shaohua Yao9Laboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan UniversityLaboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan UniversityLaboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan UniversityLaboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan UniversityLaboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan UniversityLaboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan UniversityLaboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan UniversityLaboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan UniversityLaboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan UniversityLaboratory of Biotherapy, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan UniversityAbstract We present a base editing system, in which base editors are attached to different sites of sgRNA scaffold (sgBE). Each independent sgBE has its own specific editing pattern for a given target site. Among tested sgBEs, sgBE-SL4, in which deaminase is attached to the last stem-loop of sgRNA, yields the highest editing efficiency in the window several nucleotides next to the one edited by BE3. sgBE enables the simultaneous editing of adenine and cytosine. Finally, in order to facilitate in vivo base editing, we extend our sgBE system to an AAV-compatible Cas9, SaCas9 (Staphylococcus aureus), and observe robust base editing.http://link.springer.com/article/10.1186/s13059-020-02137-6 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Yanhong Wang Lifang Zhou Rui Tao Nan Liu Jie Long Fengming Qin Wenling Tang Yang Yang Qiang Chen Shaohua Yao |
| spellingShingle |
Yanhong Wang Lifang Zhou Rui Tao Nan Liu Jie Long Fengming Qin Wenling Tang Yang Yang Qiang Chen Shaohua Yao sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine Genome Biology |
| author_facet |
Yanhong Wang Lifang Zhou Rui Tao Nan Liu Jie Long Fengming Qin Wenling Tang Yang Yang Qiang Chen Shaohua Yao |
| author_sort |
Yanhong Wang |
| title |
sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine |
| title_short |
sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine |
| title_full |
sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine |
| title_fullStr |
sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine |
| title_full_unstemmed |
sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine |
| title_sort |
sgbe: a structure-guided design of sgrna architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine |
| publisher |
BMC |
| series |
Genome Biology |
| issn |
1474-760X |
| publishDate |
2020-08-01 |
| description |
Abstract We present a base editing system, in which base editors are attached to different sites of sgRNA scaffold (sgBE). Each independent sgBE has its own specific editing pattern for a given target site. Among tested sgBEs, sgBE-SL4, in which deaminase is attached to the last stem-loop of sgRNA, yields the highest editing efficiency in the window several nucleotides next to the one edited by BE3. sgBE enables the simultaneous editing of adenine and cytosine. Finally, in order to facilitate in vivo base editing, we extend our sgBE system to an AAV-compatible Cas9, SaCas9 (Staphylococcus aureus), and observe robust base editing. |
| url |
http://link.springer.com/article/10.1186/s13059-020-02137-6 |
| work_keys_str_mv |
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