Patterns of expression of cell wall related genes in sugarcane

Our search for genes related to cell wall metabolism in the sugarcane expressed sequence tag (SUCEST) database (<A HREF="http://sucest.lbi.dcc.unicamp.br/">http://sucest.lbi.dcc.unicamp.br</A>) resulted in 3,283 reads (1% of the total reads) which were grouped into 459 clusters...

Full description

Bibliographic Details
Main Authors: Lima D.U., Santos H.P., Tiné M.A., Molle F.R.D., Buckeridge M.S.
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2001-01-01
Series:Genetics and Molecular Biology
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100026
id doaj-106e36b00d164071a53c0cbed15fd7e6
record_format Article
spelling doaj-106e36b00d164071a53c0cbed15fd7e62020-11-25T01:10:56ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852001-01-01241-4191198Patterns of expression of cell wall related genes in sugarcaneLima D.U.Santos H.P.Tiné M.A.Molle F.R.D.Buckeridge M.S.Our search for genes related to cell wall metabolism in the sugarcane expressed sequence tag (SUCEST) database (<A HREF="http://sucest.lbi.dcc.unicamp.br/">http://sucest.lbi.dcc.unicamp.br</A>) resulted in 3,283 reads (1% of the total reads) which were grouped into 459 clusters (potential genes) with an average of 7.1 reads per cluster. To more clearly display our correlation coefficients, we constructed surface maps which we used to investigate the relationship between cell wall genes and the sugarcane tissues libraries from which they came. The only significant correlations that we found between cell wall genes and/or their expression within particular libraries were neutral or synergetic. Genes related to cellulose biosynthesis were from the CesA family, and were found to be the most abundant cell wall related genes in the SUCEST database. We found that the highest number of CesA reads came from the root and stem libraries. The genes with the greatest number of reads were those involved in cell wall hydrolases (e.g. beta-1,3-glucanases, xyloglucan endo-beta-transglycosylase, beta-glucosidase and endo-beta-mannanase). Correlation analyses by surface mapping revealed that the expression of genes related to biosynthesis seems to be associated with the hydrolysis of hemicelluloses, pectin hydrolases being mainly associated with xyloglucan hydrolases. The patterns of cell wall related gene expression in sugarcane based on the number of reads per cluster reflected quite well the expected physiological characteristics of the tissues. This is the first work to provide a general view on plant cell wall metabolism through the expression of related genes in almost all the tissues of a plant at the same time. For example, developing flowers behaved similarly to both meristematic tissues and leaf-root transition zone tissues. Besides providing a basis for future research on the mechanisms of plant development which involve the cell wall, our findings will provide valuable tools for plant engineering in the near future.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100026
collection DOAJ
language English
format Article
sources DOAJ
author Lima D.U.
Santos H.P.
Tiné M.A.
Molle F.R.D.
Buckeridge M.S.
spellingShingle Lima D.U.
Santos H.P.
Tiné M.A.
Molle F.R.D.
Buckeridge M.S.
Patterns of expression of cell wall related genes in sugarcane
Genetics and Molecular Biology
author_facet Lima D.U.
Santos H.P.
Tiné M.A.
Molle F.R.D.
Buckeridge M.S.
author_sort Lima D.U.
title Patterns of expression of cell wall related genes in sugarcane
title_short Patterns of expression of cell wall related genes in sugarcane
title_full Patterns of expression of cell wall related genes in sugarcane
title_fullStr Patterns of expression of cell wall related genes in sugarcane
title_full_unstemmed Patterns of expression of cell wall related genes in sugarcane
title_sort patterns of expression of cell wall related genes in sugarcane
publisher Sociedade Brasileira de Genética
series Genetics and Molecular Biology
issn 1415-4757
1678-4685
publishDate 2001-01-01
description Our search for genes related to cell wall metabolism in the sugarcane expressed sequence tag (SUCEST) database (<A HREF="http://sucest.lbi.dcc.unicamp.br/">http://sucest.lbi.dcc.unicamp.br</A>) resulted in 3,283 reads (1% of the total reads) which were grouped into 459 clusters (potential genes) with an average of 7.1 reads per cluster. To more clearly display our correlation coefficients, we constructed surface maps which we used to investigate the relationship between cell wall genes and the sugarcane tissues libraries from which they came. The only significant correlations that we found between cell wall genes and/or their expression within particular libraries were neutral or synergetic. Genes related to cellulose biosynthesis were from the CesA family, and were found to be the most abundant cell wall related genes in the SUCEST database. We found that the highest number of CesA reads came from the root and stem libraries. The genes with the greatest number of reads were those involved in cell wall hydrolases (e.g. beta-1,3-glucanases, xyloglucan endo-beta-transglycosylase, beta-glucosidase and endo-beta-mannanase). Correlation analyses by surface mapping revealed that the expression of genes related to biosynthesis seems to be associated with the hydrolysis of hemicelluloses, pectin hydrolases being mainly associated with xyloglucan hydrolases. The patterns of cell wall related gene expression in sugarcane based on the number of reads per cluster reflected quite well the expected physiological characteristics of the tissues. This is the first work to provide a general view on plant cell wall metabolism through the expression of related genes in almost all the tissues of a plant at the same time. For example, developing flowers behaved similarly to both meristematic tissues and leaf-root transition zone tissues. Besides providing a basis for future research on the mechanisms of plant development which involve the cell wall, our findings will provide valuable tools for plant engineering in the near future.
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100026
work_keys_str_mv AT limadu patternsofexpressionofcellwallrelatedgenesinsugarcane
AT santoshp patternsofexpressionofcellwallrelatedgenesinsugarcane
AT tinema patternsofexpressionofcellwallrelatedgenesinsugarcane
AT mollefrd patternsofexpressionofcellwallrelatedgenesinsugarcane
AT buckeridgems patternsofexpressionofcellwallrelatedgenesinsugarcane
_version_ 1725173397588017152