Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain after Intra-arterial Infusion in Rats

Intra-arterial (IA) delivery of bone marrow-derived mesenchymal stem cells (BM-MSCs) has shown potential as a minimally invasive therapeutic approach for stroke. The aim of the present study was to determine the whole-body biodistribution and clearance of technetium-99m ( 99m Tc)-labeled rat and hum...

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Main Authors: Joonas Khabbal, Erja Kerkelä, Bhimashankar Mitkari, Mari Raki, Johanna Nystedt, Ville Mikkonen, Kim Bergström, Saara Laitinen, Matti Korhonen, Jukka Jolkkonen Ph.D.
Format: Article
Language:English
Published: SAGE Publishing 2015-05-01
Series:Cell Transplantation
Online Access:https://doi.org/10.3727/096368914X679336
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spelling doaj-10490bcae907475e9b0c31c3e8168c162020-11-25T03:52:03ZengSAGE PublishingCell Transplantation0963-68971555-38922015-05-012410.3727/096368914X679336Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain after Intra-arterial Infusion in RatsJoonas Khabbal0Erja Kerkelä1Bhimashankar Mitkari2Mari Raki3Johanna Nystedt4Ville Mikkonen5Kim Bergström6Saara Laitinen7Matti Korhonen8Jukka Jolkkonen Ph.D.9 Institute of Clinical Medicine - Neurology, University of Eastern Finland, Kuopio, Finland Finnish Red Cross Blood Service, Advanced Therapies and Product Development, Helsinki, Finland Institute of Clinical Medicine - Neurology, University of Eastern Finland, Kuopio, Finland Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland Finnish Red Cross Blood Service, Advanced Therapies and Product Development, Helsinki, Finland Institute of Clinical Medicine - Neurology, University of Eastern Finland, Kuopio, Finland HUS Medical Imaging Centre, Helsinki University Central Hospital, Helsinki, Finland Finnish Red Cross Blood Service, Advanced Therapies and Product Development, Helsinki, Finland Finnish Red Cross Blood Service, Advanced Therapies and Product Development, Helsinki, Finland Institute of Clinical Medicine - Neurology, University of Eastern Finland, Kuopio, FinlandIntra-arterial (IA) delivery of bone marrow-derived mesenchymal stem cells (BM-MSCs) has shown potential as a minimally invasive therapeutic approach for stroke. The aim of the present study was to determine the whole-body biodistribution and clearance of technetium-99m ( 99m Tc)-labeled rat and human BM-MSCs after IA delivery in a rat model of transient middle cerebral artery occlusion (MCAO) using single-photon emission computed tomography (SPECT). Our hypothesis was that xenotransplantation has a major impact on the behavior of cells. Male RccHan: Wistar rats were subjected to sham operation or MCAO. Twenty-four hours after surgery, BM-MSCs (2×10 6 cells/animal) labeled with 99m Tc were infused into the external carotid artery. Whole-body SPECT images were acquired 20 min, 3 h, and 6 h postinjection, after which rats were sacrificed, and organs were collected and weighed for measurement of radioactivity. The results showed that the majority of the cells were located in the brain and especially in the ipsilateral hemisphere immediately after cell infusion both in sham-operated and MCAO rats. This was followed by fast disappearance, particularly in the case of human cells. At the same time, the radioactivity signal increased in the spleen, kidney, and liver, the organs responsible for destroying cells. Further studies are needed to demonstrate whether differential cell behavior has any functional impact.https://doi.org/10.3727/096368914X679336
collection DOAJ
language English
format Article
sources DOAJ
author Joonas Khabbal
Erja Kerkelä
Bhimashankar Mitkari
Mari Raki
Johanna Nystedt
Ville Mikkonen
Kim Bergström
Saara Laitinen
Matti Korhonen
Jukka Jolkkonen Ph.D.
spellingShingle Joonas Khabbal
Erja Kerkelä
Bhimashankar Mitkari
Mari Raki
Johanna Nystedt
Ville Mikkonen
Kim Bergström
Saara Laitinen
Matti Korhonen
Jukka Jolkkonen Ph.D.
Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain after Intra-arterial Infusion in Rats
Cell Transplantation
author_facet Joonas Khabbal
Erja Kerkelä
Bhimashankar Mitkari
Mari Raki
Johanna Nystedt
Ville Mikkonen
Kim Bergström
Saara Laitinen
Matti Korhonen
Jukka Jolkkonen Ph.D.
author_sort Joonas Khabbal
title Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain after Intra-arterial Infusion in Rats
title_short Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain after Intra-arterial Infusion in Rats
title_full Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain after Intra-arterial Infusion in Rats
title_fullStr Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain after Intra-arterial Infusion in Rats
title_full_unstemmed Differential Clearance of Rat and Human Bone Marrow-Derived Mesenchymal Stem Cells from the Brain after Intra-arterial Infusion in Rats
title_sort differential clearance of rat and human bone marrow-derived mesenchymal stem cells from the brain after intra-arterial infusion in rats
publisher SAGE Publishing
series Cell Transplantation
issn 0963-6897
1555-3892
publishDate 2015-05-01
description Intra-arterial (IA) delivery of bone marrow-derived mesenchymal stem cells (BM-MSCs) has shown potential as a minimally invasive therapeutic approach for stroke. The aim of the present study was to determine the whole-body biodistribution and clearance of technetium-99m ( 99m Tc)-labeled rat and human BM-MSCs after IA delivery in a rat model of transient middle cerebral artery occlusion (MCAO) using single-photon emission computed tomography (SPECT). Our hypothesis was that xenotransplantation has a major impact on the behavior of cells. Male RccHan: Wistar rats were subjected to sham operation or MCAO. Twenty-four hours after surgery, BM-MSCs (2×10 6 cells/animal) labeled with 99m Tc were infused into the external carotid artery. Whole-body SPECT images were acquired 20 min, 3 h, and 6 h postinjection, after which rats were sacrificed, and organs were collected and weighed for measurement of radioactivity. The results showed that the majority of the cells were located in the brain and especially in the ipsilateral hemisphere immediately after cell infusion both in sham-operated and MCAO rats. This was followed by fast disappearance, particularly in the case of human cells. At the same time, the radioactivity signal increased in the spleen, kidney, and liver, the organs responsible for destroying cells. Further studies are needed to demonstrate whether differential cell behavior has any functional impact.
url https://doi.org/10.3727/096368914X679336
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