Effect of hydroxypropyl methyl cellulose phthalate coating on digestive stability and intestinal transport of green tea catechins

• Main Authors: Jae-Hwan Chung, Sang-Jun Lee, Jin-Oh Chung, Yu-Jin Oh, Jeong-Ah Hwang, Young-Kyung Kim, Sanghoon Ko, Soon-Mi Shim
• Format: Article
• Language: English
• Published: Elsevier 2014-03-01
• Series: Integrative Medicine Research
• Subjects: digestive stability; green tea catechin; hydroxypropyl methyl cellulose phthalate; intestinal transport
• Online Access: http://www.sciencedirect.com/science/article/pii/S2213422013000978

Background: The purpose of this study was to investigate the effect of hydroxypropyl methyl cellulose phthalate (HPMCP) coating on the digestive stability and intestinal transport of green tea catechins (GTCs). Methods: Two types of HPMCP coating were prepared: one type with size smaller than 500 μm (S-HPMCP) and the other with size larger than 500 μm (L-HPMCP). An in vitro gastrointestinal model system coupled with Caco-2 cells was used for estimating the bioavailability of GTCs. Ultraperformance liquid chromatography with a photodiode array detector was performed to analyze GTCs. Results: The digestive stability of GTCs was enhanced up to 33.73% and 35.28% for S-HPMCP and L-HPMCP, respectively. Intestinal transport of the GTCs was increased to 22.98% and 23.23% for S-HPMCP and L-HPMCP, respectively. Overall, the bioavailability of GTCs increased by 4.08 and 11.71 times for S-HPMCP and L-HPMCP, respectively. Conclusion: The results of this study confirm that coating with HPMCP could be a way to improve the digestive stability and intestinal transport of GTCs.