Effect of water hardness/alkalinity and humic substances on the toxicity of peracetic acid to zebrafish embryos and pathogenic isolates

The prophylactic use of peracetic acid (PAA)-based disinfectants is becoming more popular in aquaculture due to rising concerns regarding sustainability, fish welfare and food safety. However, specific and effective PAA dosing protocols have not been developed to guide the aquaculture industry under...

Full description

Bibliographic Details
Main Authors: Dibo Liu, Anna Marrodán Pellicer, Anneliese Brüggmann, Mirijam Kiggen, Sascha Behrens, Christopher Good, David L. Straus, Thomas Meinelt
Format: Article
Language:English
Published: Elsevier 2021-11-01
Series:Aquaculture Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352513421003161
id doaj-103851a9fbd040e1a18f9cb7b92badeb
record_format Article
spelling doaj-103851a9fbd040e1a18f9cb7b92badeb2021-10-09T04:39:46ZengElsevierAquaculture Reports2352-51342021-11-0121100900Effect of water hardness/alkalinity and humic substances on the toxicity of peracetic acid to zebrafish embryos and pathogenic isolatesDibo Liu0Anna Marrodán Pellicer1Anneliese Brüggmann2Mirijam Kiggen3Sascha Behrens4Christopher Good5David L. Straus6Thomas Meinelt7Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 301, 12587 Berlin, Germany; Corresponding author.Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Faculty of Life Sciences, Humboldt University of Berlin, Invalidenstraße 42, 10115 Berlin, GermanyAlbrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Faculty of Life Sciences, Humboldt University of Berlin, Invalidenstraße 42, 10115 Berlin, GermanyAlbrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Faculty of Life Sciences, Humboldt University of Berlin, Invalidenstraße 42, 10115 Berlin, GermanyDepartment of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 301, 12587 Berlin, GermanyThe Conservation Fund Freshwater Institute, 1098 Turner Road, Shepherdstown, WV, USAUS Department of Agriculture, Agriculural Research Service, Harry K. Dupree—Stuttgart National Aquaculture Research Center, P.O. Box 1050, Stuttgart, AR 72160, USADepartment of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 301, 12587 Berlin, GermanyThe prophylactic use of peracetic acid (PAA)-based disinfectants is becoming more popular in aquaculture due to rising concerns regarding sustainability, fish welfare and food safety. However, specific and effective PAA dosing protocols have not been developed to guide the aquaculture industry under diverse production conditions. In the present study, the effect of water hardness/alkalinity and humic substances (HS) on the toxicity of PAA to zebrafish Danio rerio embryos and the efficacy of PAA against the in vitro growth of Yersinia ruckeri and Saprolegnia parasitica was investigated. PAA concentrations that were safe to fish embryos demonstrated strong bactericidal, but limited fungistatic properties. In higher hardness/alkalinity water, or when HS was added, the same concentration of PAA resulted in a smaller pH decrease accompanied by a smaller increase of oxidation-reduction potential (ORP), and showed lower toxicity and weaker antimicrobial effects than in lower hardness/alkalinity waters. We suggest the determining factor of PAA toxicity and its antimicrobial capacity was likely ORP. At low hardness/alkalinity conditions, strong pH reduction (resulting in pH<5) was the dominant role in PAA toxicity to D. rerio embryos. In aquaculture settings, lower PAA doses should be used under lower hardness/alkalinity conditions. Addition of HS under lower hardness/alkalinity conditions can assist with reducing toxicity and the risk to fish. Finally, we determined that repeated PAA disinfection is necessary to achieve a sustained prophylaxis, and we caution that the instant formation of aggregates by HS at high hardness conditions and the subsequent attachment of bacteria may reduce their susceptibility to PAA disinfection.http://www.sciencedirect.com/science/article/pii/S2352513421003161ZebrafishY. ruckeriS. parasiticaOxidation-reduction potentialSustainable aquaculture
collection DOAJ
language English
format Article
sources DOAJ
author Dibo Liu
Anna Marrodán Pellicer
Anneliese Brüggmann
Mirijam Kiggen
Sascha Behrens
Christopher Good
David L. Straus
Thomas Meinelt
spellingShingle Dibo Liu
Anna Marrodán Pellicer
Anneliese Brüggmann
Mirijam Kiggen
Sascha Behrens
Christopher Good
David L. Straus
Thomas Meinelt
Effect of water hardness/alkalinity and humic substances on the toxicity of peracetic acid to zebrafish embryos and pathogenic isolates
Aquaculture Reports
Zebrafish
Y. ruckeri
S. parasitica
Oxidation-reduction potential
Sustainable aquaculture
author_facet Dibo Liu
Anna Marrodán Pellicer
Anneliese Brüggmann
Mirijam Kiggen
Sascha Behrens
Christopher Good
David L. Straus
Thomas Meinelt
author_sort Dibo Liu
title Effect of water hardness/alkalinity and humic substances on the toxicity of peracetic acid to zebrafish embryos and pathogenic isolates
title_short Effect of water hardness/alkalinity and humic substances on the toxicity of peracetic acid to zebrafish embryos and pathogenic isolates
title_full Effect of water hardness/alkalinity and humic substances on the toxicity of peracetic acid to zebrafish embryos and pathogenic isolates
title_fullStr Effect of water hardness/alkalinity and humic substances on the toxicity of peracetic acid to zebrafish embryos and pathogenic isolates
title_full_unstemmed Effect of water hardness/alkalinity and humic substances on the toxicity of peracetic acid to zebrafish embryos and pathogenic isolates
title_sort effect of water hardness/alkalinity and humic substances on the toxicity of peracetic acid to zebrafish embryos and pathogenic isolates
publisher Elsevier
series Aquaculture Reports
issn 2352-5134
publishDate 2021-11-01
description The prophylactic use of peracetic acid (PAA)-based disinfectants is becoming more popular in aquaculture due to rising concerns regarding sustainability, fish welfare and food safety. However, specific and effective PAA dosing protocols have not been developed to guide the aquaculture industry under diverse production conditions. In the present study, the effect of water hardness/alkalinity and humic substances (HS) on the toxicity of PAA to zebrafish Danio rerio embryos and the efficacy of PAA against the in vitro growth of Yersinia ruckeri and Saprolegnia parasitica was investigated. PAA concentrations that were safe to fish embryos demonstrated strong bactericidal, but limited fungistatic properties. In higher hardness/alkalinity water, or when HS was added, the same concentration of PAA resulted in a smaller pH decrease accompanied by a smaller increase of oxidation-reduction potential (ORP), and showed lower toxicity and weaker antimicrobial effects than in lower hardness/alkalinity waters. We suggest the determining factor of PAA toxicity and its antimicrobial capacity was likely ORP. At low hardness/alkalinity conditions, strong pH reduction (resulting in pH<5) was the dominant role in PAA toxicity to D. rerio embryos. In aquaculture settings, lower PAA doses should be used under lower hardness/alkalinity conditions. Addition of HS under lower hardness/alkalinity conditions can assist with reducing toxicity and the risk to fish. Finally, we determined that repeated PAA disinfection is necessary to achieve a sustained prophylaxis, and we caution that the instant formation of aggregates by HS at high hardness conditions and the subsequent attachment of bacteria may reduce their susceptibility to PAA disinfection.
topic Zebrafish
Y. ruckeri
S. parasitica
Oxidation-reduction potential
Sustainable aquaculture
url http://www.sciencedirect.com/science/article/pii/S2352513421003161
work_keys_str_mv AT diboliu effectofwaterhardnessalkalinityandhumicsubstancesonthetoxicityofperaceticacidtozebrafishembryosandpathogenicisolates
AT annamarrodanpellicer effectofwaterhardnessalkalinityandhumicsubstancesonthetoxicityofperaceticacidtozebrafishembryosandpathogenicisolates
AT anneliesebruggmann effectofwaterhardnessalkalinityandhumicsubstancesonthetoxicityofperaceticacidtozebrafishembryosandpathogenicisolates
AT mirijamkiggen effectofwaterhardnessalkalinityandhumicsubstancesonthetoxicityofperaceticacidtozebrafishembryosandpathogenicisolates
AT saschabehrens effectofwaterhardnessalkalinityandhumicsubstancesonthetoxicityofperaceticacidtozebrafishembryosandpathogenicisolates
AT christophergood effectofwaterhardnessalkalinityandhumicsubstancesonthetoxicityofperaceticacidtozebrafishembryosandpathogenicisolates
AT davidlstraus effectofwaterhardnessalkalinityandhumicsubstancesonthetoxicityofperaceticacidtozebrafishembryosandpathogenicisolates
AT thomasmeinelt effectofwaterhardnessalkalinityandhumicsubstancesonthetoxicityofperaceticacidtozebrafishembryosandpathogenicisolates
_version_ 1716830578559418368