Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease

• Main Authors: Arezoo Haghighi, Zivar Salehi, Keyvan Aminian, saba Fakhrieh Asl
• Format: Article
• Language: fas
• Published: Birjand University of Medical Sciences and Health Services 2014-05-01
• Series: Journal of Birjand University of Medical Sciences
• Subjects: Peptic ulcer disease; Mitochondria genome
• Online Access: http://journal.bums.ac.ir/browse.php?a_code=A-10-1206-1&slc_lang=en&sid=1

Background and Aim: A peptic ulcer is a breach in the gastric or duodenal mucosa down to the submucosa. There is evidence concerning the role of Reactive Oxygen Species (ROS) in the genesis of such ulcers production of intracellular ROS along mitochondria oxidative phosphorylation (OXPHOS) predisposes the deletion of 4977 bp mtDNA. The aim of the present study was to evaluate the association of 4977 bp mtDNA deletion with peptic ulcer disease. Materials and Methods: In this case-control study included 110 patients with peptic ulcer disease and 110 healthy individuals were compared. Genomic DNAs of the cases and controls were extracted from bioptic tissues. Then, their genotypes were determined by means of Polymerase Chain Reaction (PCR). Finally, statistical analysis was performed using the MedCalc program. Results: Deletion of 4977 bp mtDNA was found to be more frequent among patients with peptic ulcer disease (52.7%) compared to the controls (15.3%). A significant association was found between the deletion with peptic ulcer disease. Conclusion: Deletion of 4977 bp in mitochondrial DNA is associated with peptic ulcer disease.