A Simple Breeding Protocol for the Procurement of Accurately Staged Rat Donor Embryos for Neural Transplantation

A Simple Breeding Protocol for the Procurement of Accurately Staged Rat Donor Embryos for Neural Transplantation

Obtaining accurately staged rat embryos can be difficult because of the variety of breeding protocols employed and because precise staging cannot be confirmed until excision of the embryos from the dam. The detection of estrus, pairing of animals, and confirmation of pregnancies is generally left to...

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Main Authors: U. M. Weyrauch, E. M. Torres, A. L. Baird, S. B. Dunnett
Format: Article
Language:English
Published: SAGE Publishing 2009-04-01
Series:Cell Transplantation
Online Access:https://doi.org/10.3727/096368909788809802
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spelling doaj-1030bc3d7dff423eb3a03419923f0f9b2020-11-25T03:15:10ZengSAGE PublishingCell Transplantation0963-68971555-38922009-04-011810.3727/096368909788809802A Simple Breeding Protocol for the Procurement of Accurately Staged Rat Donor Embryos for Neural TransplantationU. M. Weyrauch0E. M. Torres1A. L. Baird2S. B. Dunnett3Department of Biosciences, Cardiff University, Cardiff, UKDepartment of Biosciences, Cardiff University, Cardiff, UKDepartment of Biosciences, Cardiff University, Cardiff, UKDepartment of Biosciences, Cardiff University, Cardiff, UKObtaining accurately staged rat embryos can be difficult because of the variety of breeding protocols employed and because precise staging cannot be confirmed until excision of the embryos from the dam. The detection of estrus, pairing of animals, and confirmation of pregnancies is generally left to commercial suppliers, as in-house breeding can be laborious and unpredictable. Here we describe a simple, reliable in-house breeding protocol for the generation of accurately staged embryos as assessed by measurements of average crown to rump length (CRL).https://doi.org/10.3727/096368909788809802
collection DOAJ
language English
format Article
sources DOAJ
author U. M. Weyrauch
E. M. Torres
A. L. Baird
S. B. Dunnett
spellingShingle U. M. Weyrauch
E. M. Torres
A. L. Baird
S. B. Dunnett
A Simple Breeding Protocol for the Procurement of Accurately Staged Rat Donor Embryos for Neural Transplantation
Cell Transplantation
author_facet U. M. Weyrauch
E. M. Torres
A. L. Baird
S. B. Dunnett
author_sort U. M. Weyrauch
title A Simple Breeding Protocol for the Procurement of Accurately Staged Rat Donor Embryos for Neural Transplantation
title_short A Simple Breeding Protocol for the Procurement of Accurately Staged Rat Donor Embryos for Neural Transplantation
title_full A Simple Breeding Protocol for the Procurement of Accurately Staged Rat Donor Embryos for Neural Transplantation
title_fullStr A Simple Breeding Protocol for the Procurement of Accurately Staged Rat Donor Embryos for Neural Transplantation
title_full_unstemmed A Simple Breeding Protocol for the Procurement of Accurately Staged Rat Donor Embryos for Neural Transplantation
title_sort simple breeding protocol for the procurement of accurately staged rat donor embryos for neural transplantation
publisher SAGE Publishing
series Cell Transplantation
issn 0963-6897
1555-3892
publishDate 2009-04-01
description Obtaining accurately staged rat embryos can be difficult because of the variety of breeding protocols employed and because precise staging cannot be confirmed until excision of the embryos from the dam. The detection of estrus, pairing of animals, and confirmation of pregnancies is generally left to commercial suppliers, as in-house breeding can be laborious and unpredictable. Here we describe a simple, reliable in-house breeding protocol for the generation of accurately staged embryos as assessed by measurements of average crown to rump length (CRL).
url https://doi.org/10.3727/096368909788809802
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