Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection
In this paper, a novel method for interaction detection is presented to compare the contact dynamics of macrophages in the <i>Drosophila</i> embryo. The study is carried out by a framework called macrosight, which analyses the movement and interaction of migrating macrophages. The framew...
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MDPI AG
2020-05-01
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| Series: | Journal of Imaging |
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| Online Access: | https://www.mdpi.com/2313-433X/6/5/36 |
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doaj-101518eb98a445cda9f77a6450a19b5b2020-11-25T03:03:13ZengMDPI AGJournal of Imaging2313-433X2020-05-016363610.3390/jimaging6050036Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction DetectionJosé Alonso Solís-Lemus0Besaiz J Sánchez-Sánchez1Stefania Marcotti2Mubarik Burki3Brian Stramer4Constantino Carlos Reyes-Aldasoro5School of Biomedical Engineering and Imaging Sciences, King’s College London, London SE1 7EH, UKRandall Centre for Cell & Molecular Biophysics, King’s College London, London SE1 1UL, UKRandall Centre for Cell & Molecular Biophysics, King’s College London, London SE1 1UL, UKRandall Centre for Cell & Molecular Biophysics, King’s College London, London SE1 1UL, UKRandall Centre for Cell & Molecular Biophysics, King’s College London, London SE1 1UL, UKGiCentre, Departmen t of Computer Science, School of Mathematics, Computer Science and Engineering, City, University of London, London EC1V 0HB, UKIn this paper, a novel method for interaction detection is presented to compare the contact dynamics of macrophages in the <i>Drosophila</i> embryo. The study is carried out by a framework called macrosight, which analyses the movement and interaction of migrating macrophages. The framework incorporates a segmentation and tracking algorithm into analysing the motion characteristics of cells after contact. In this particular study, the interactions between cells is characterised in the case of control embryos and <i>Shot</i> mutants, a candidate protein that is hypothesised to regulate contact dynamics between migrating cells. Statistical significance between control and mutant cells was found when comparing the direction of motion after contact in specific conditions. Such discoveries provide insights for future developments in combining biological experiments with computational analysis.https://www.mdpi.com/2313-433X/6/5/36cell segmentationcell trackingmacrophagescell shapecontact analysis |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
José Alonso Solís-Lemus Besaiz J Sánchez-Sánchez Stefania Marcotti Mubarik Burki Brian Stramer Constantino Carlos Reyes-Aldasoro |
| spellingShingle |
José Alonso Solís-Lemus Besaiz J Sánchez-Sánchez Stefania Marcotti Mubarik Burki Brian Stramer Constantino Carlos Reyes-Aldasoro Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection Journal of Imaging cell segmentation cell tracking macrophages cell shape contact analysis |
| author_facet |
José Alonso Solís-Lemus Besaiz J Sánchez-Sánchez Stefania Marcotti Mubarik Burki Brian Stramer Constantino Carlos Reyes-Aldasoro |
| author_sort |
José Alonso Solís-Lemus |
| title |
Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection |
| title_short |
Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection |
| title_full |
Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection |
| title_fullStr |
Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection |
| title_full_unstemmed |
Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection |
| title_sort |
comparative study of contact repulsion in control and mutant macrophages using a novel interaction detection |
| publisher |
MDPI AG |
| series |
Journal of Imaging |
| issn |
2313-433X |
| publishDate |
2020-05-01 |
| description |
In this paper, a novel method for interaction detection is presented to compare the contact dynamics of macrophages in the <i>Drosophila</i> embryo. The study is carried out by a framework called macrosight, which analyses the movement and interaction of migrating macrophages. The framework incorporates a segmentation and tracking algorithm into analysing the motion characteristics of cells after contact. In this particular study, the interactions between cells is characterised in the case of control embryos and <i>Shot</i> mutants, a candidate protein that is hypothesised to regulate contact dynamics between migrating cells. Statistical significance between control and mutant cells was found when comparing the direction of motion after contact in specific conditions. Such discoveries provide insights for future developments in combining biological experiments with computational analysis. |
| topic |
cell segmentation cell tracking macrophages cell shape contact analysis |
| url |
https://www.mdpi.com/2313-433X/6/5/36 |
| work_keys_str_mv |
AT josealonsosolislemus comparativestudyofcontactrepulsionincontrolandmutantmacrophagesusinganovelinteractiondetection AT besaizjsanchezsanchez comparativestudyofcontactrepulsionincontrolandmutantmacrophagesusinganovelinteractiondetection AT stefaniamarcotti comparativestudyofcontactrepulsionincontrolandmutantmacrophagesusinganovelinteractiondetection AT mubarikburki comparativestudyofcontactrepulsionincontrolandmutantmacrophagesusinganovelinteractiondetection AT brianstramer comparativestudyofcontactrepulsionincontrolandmutantmacrophagesusinganovelinteractiondetection AT constantinocarlosreyesaldasoro comparativestudyofcontactrepulsionincontrolandmutantmacrophagesusinganovelinteractiondetection |
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