Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant

• Main Authors: Ali A. Taha, Nahida J. Hameed, Farah Hamed Ali
• Format: Article
• Language: Arabic
• Published: Al-Mustansiriyah University 2020-08-01
• Series: Mustansiriyah Journal of Science
• Subjects: polycyclic aromatic hydrocarbons pah; immobilized laccase; trametes versicolor; bioremediation; hbt; conversation; hyacinth plant.
• Online Access: http://mjs.uomustansiriyah.edu.iq/ojs1/index.php/MJS/article/view/670

Polycyclic aromatic hydrocarbons (PAHs) are recognized as a toxic, mutagenic and/or carcinogenic compounds, and their pollution of soil and aquifer is of increasing environmentally risk. Laccases (E.C. 1.10.3.2) are phenoloxidases catalyze the oxidation of PAHs in the presence of a mediator compound and hyacinth plant. In this study laccase from Trametes versicolor was immobilized into chitosan, and the potential to oxidize anthracene in the presence of 1-hydroxybenzotriazole (HBT) was examined. Results indicated that the immobilization enhanced the stability of laccase against temperature, pH, inhibitors and loading time compared with the other cases. The immobilized laccase-mediator system was as efficient as the free enzyme for oxidizing the tested PAHs. After 24h. of incubation, immobilized laccase–HBT showed a system oxidization more than immobilized laccase without (HBT) of PAHs; Chitosan with hyacinth plant and (HBT) resulted better conversion than chitosan with or without HBT. These results indicate a new chance for applying the immobilized laccase in bioremediation.